窒素固定菌に関する研究(第1報) : 好気性窒素固定菌の分離およびその菌学的性質

概要

論文の詳細を見る
Twenry-seven strains of nitrogen-fixing microorganisms, capable of strongly fixing atmospheric nitrogen were isolated from 837 samples taken from sewage, paddy-fields and soils of Aichi-ken, Japan. Two strains of them, A-400 and W-290,could fix nitrogen more strongly than others, and were selected as the orgenisms used in this experiment.Strain A-400 and strain W-290 were isolated from the soil of a pear orchard and the mud of a paddy-field, respectively.The Medium used for isolation was a nitrogen-free mineral salts medium with 1% glucose. The mineral salts medium consisted of K_3HPO_4 0.5g, MgSO_4・7H_2O 0.35g, NaCl0.2g, CaCl_2・H_4O 0.1g, FeSO_4・7H_2O 0.01g, MnSO_4・H_2O 0.005g and Na_2MoO_4・2H_2O(10^<-6>M) lml per litre. While a 2% glucose medium without MnSO_4・H_2O was used in the experiments for determining physiological properties.Tests for identification were based on the methods described in the Manual of Microbiological Methods. According to Bergey's Manual of Determinative Bacteriology, W-290 might be identified to be a strain of Azotobacter chroococcum. A-400 could liquefy galatin, but was unable to utilize mannitol and change litmus milk. These properties were quite different from those of the species described in Bergey's Manual. Therefore, A-400 could be considered to be a new species of Azotobacter.
社団法人日本生物工学会の論文
1963-09-15