麹菌の生産する蛍光性物質に関する研究 : (第8報)Asperopterin Bの精製単離とBおよびAの化学的性質

概要

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A new pterin, Asperopterin B (abbreviated to B), which is a bluish purple fluorescent product of Aspergillus fungi, was extracted and isolated in a crystalline form from a culture broth of Aspergillus oryzae T-17 together with Asperopterin A (abbreviated to A). Chemical properties A and B were studied. A was shown to be a glycoside pterin derivative and was hydrolyzed by acid and fractionated into a sugar moiety and aglycon pterin. The former was identified as ribose and its benzylphenylhydrazone precisely coincided with that prepared from D-ribopyranose in the IR spectra and X-ray diffraction pattern, suggesting to have D-configuration. A study on the properties of B proved B was identical with the aglycon of A. A consisted of one mole of B and one mole of ribose, the reducing end of which was bound to the aglycon. B had molecular formula C_8H_9N_5O_3・1/2H_2O. Its partial structure was investigated and defined as a derivative of 2-amino-4,7-dihydroxypteridine, by the UV and IR spectra, an elementary analysis, its reaction against AgNO_3,comparison with synthetic pterins, chelation reaction on Cu^<2+> and hot alkali decomposition. As substituents for- C_2H_6O, the -CH_2OH side chain was evidenced to exist at C_6 of the pteridine skeleton and -CH_3 was elsewhere. This was evidenced by alkaline KMnO_4 oxidation, Al-Hg reduction and others.
社団法人日本生物工学会の論文
1969-01-25