腐敗細菌のα-ケトグルタール酸-ロイシン系トランスアミナーゼ-に依るL-グルタミン酸の生成について(第1報) : 腐敗細菌のα-ケトグルタール酸-ロイシン系トランスアミナーゼの分布

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Although the α-ketoglutaric (α-KG)-leucine transaminase activity of saprogenic bacteria such as genera Flavobacterium, Alcaligenes and Achromobacter has already been reported to be high^<1)>, it is of interest to obtain more detailed information concerning the distribution of the α-KG-leucine transaminase activity as one of the chemical activities exerted by saprogenic bacteria (genera Pseudomonas, Chromobacterium, Alcaligenes, Aerobacter, Serratia, Proteus, Bacillus, Flavobacterium, Micrococcus and Brevibacterium).one hundred and forty strains belonging to the above genera were employed in our experiments. The results indicated that this transaminase activity is widely distributed in these bacteria and that the activities of some strains belonging to the genera Flavo-bacterium, Alcaligenes and Proteus are especially higher than those of the others.The cultivaiton medium containing living cells of each bacterium was directly used as the enzyme source of α-KG-leucine transaminase in these experiments. Generally, it is considered that α-KG as substrate and L-glutamic acid formed would be decomposed, as the living cells of the microorganisms contain amino acid dehydrogenase, amino acid decarboxylase, amino acid racemase and α-KG oxidase, and thus, the accumulation of L-glutamic acid would be lower, even if α-KG-leucine transaminase of these bacteria is high.The results of experimnets, wherein the decomposition of substrates and products were checked, showed that the extent of decomposition of the the substrates and products was negligibly small in the case of bacteria such as Flavobacterium fuscum, Flav. rhenanus (F-11) and Alcaligenes viscolactis (AMU-14), and that the yield of L-glutamic acid was reached to approximately 50〜60% of α-KG added.1) KatagiriHH., Tochihara T., Oshima K. and Fujii T. : Presented at the Annual Meeting of Agric. Chem. Soc. Japan, held in April (1958).
社団法人日本生物工学会の論文
1961-07-15

腐敗細菌のα-ケトグルタール酸-ロイシン系トランスアミナーゼ-に依るL-グルタミン酸の生成について(第1報) : 腐敗細菌のα-ケトグルタール酸-ロイシン系トランスアミナーゼの分布

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