酵母の生産に関する研究 : (第2報) メタノール資化性酵母Kloeckera sp. No. 2201の培養

概要

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Using a methanol assimilating yeast, Kloeckera sp. No. 2201,the cell grouth and metabolites in a methanol medium were investigated. The pH of the medium was regulated by addition of 0.2% sodium acetate after a 24 hrs. cultivation. In a study on the effect of the methanol concentration on the cell growth under favorable conditions, the cell yield was 0.225g with 1% methanol and 0.460g with 3% methanol per 100ml of broth. However, the growth was inhibited by 10% methanol. The strain assimilated ethanol and glycerol much better than carbohydrate. The cell composition was as follows; crude protein 45.30%, crude fat 5.24%, crude fiber 4.84%, nitrogen-free extract 33.67%, ash 6.90% and moisture 4.05%. Amino acid in the cells was analyzed by the amino acid autoanalyzer. As a metabolite of methanol, form-aldehyde was isolated from the cultured broth and identified as 2,4-dinitrophenylydrazone by physico-chemical methods.
社団法人日本生物工学会の論文
1970-08-25