CDP-コリン醗酵におけるコリンとCMPのリン酸化 : 酵母によるCDP-コリンの醗酵生産(V)

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An improved method for CDP-choline production in high yeilds from CMP and choline was investigated.Fructose 1,6-diphosphate (FDP) was a more favorable energy source than glucose for CDP-choline production and for the phosphorylation of both choline and CMP under the fermentation conditions. For example, in the reaction system using FDP as an energy source, the yield of CDP-choline within a short period of incubation was much higher than that in the system using glucose. The same results were observed in the phosphorylation of CMP and choline.Phosphorylation of CMP did not compete with the phosphorylation of choline in their energy requirement.Biological activities of Hansenula jadinii IFO 0987 with regard to high yields of CDP-choline production were sutdies. H. jadinii exhibited high activity of phosphorylation of CMP to CTP under the fermentation conditions. More than 40μmol per ml of CMP were phosphorylated within two hours incubation by 100 mg per ml of the dried cells. The organism also showed high activity of degradation of inorganic pyrophosphate. Two hundred and forty μmol of inorganic pyrophosphate were hydrolysed by one mg of cell per hour.Choline kinase was purified about seventy fold from the crude extract from H. jadinii. Phosphorylation of choline was greatly inhibited by 10^<-3> M of CTP. By feeding a low concentration of CMP and glucose the concentration of CTP accumulated in the reaction mixture could be maintained below the level at which choline kinase was inhibited. By use of this method, more than 33 μmol per ml of CDP-choline were produced from the total concentration of 50μmol of CMP per ml.
社団法人日本生物工学会の論文
1975-05-25

CDP-コリン醗酵におけるコリンとCMPのリン酸化 : 酵母によるCDP-コリンの醗酵生産(V)

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