酵母によるCMPとコリンからCDP-コリンの生産

概要

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Fermentative production of CDP-choline by yeasts from CMP and choline was investigated. By use of a dried cell preparation of Hansenula jadivii IFO 0987,CDP-choline was produced in good yields at high levels of inorganic phosphate under the condition of glucose catabolism. CDP-choline was isolated as its sodium salt by ion exchange column chromatography, and identified by chromatogrphic and phusoco-chemical procedures. CDP-choline production by H. jadinii was strictly controlled by broth cultivation time of cells and pH of the relaction mixture. Long time cultivation resulted in lowering the activity of both choline kinase and CDP-choline pyrophosphorylase of the cells, while the phosphorylation of CMP was not affected by long time cultivation. The optimum conditions for CDP-choline production were as follows : CMP, 20μmoles/ml; choline, 80 μmoles/ml; glucose, 600 μmoles/ml; MgSO_4・7H_2O, 30μmoles/ml and dried cell preparation 100-200 mg/ml in total volume of 2 ml. Under the optimum conditions, the maximum yield of CDP-choline was more than 13 μmoles per ml.
社団法人日本生物工学会の論文
1974-09-25