麹菌のプロテアーゼの生成について (第2報) : Inactive proteaseの生活化因子に関する検討

概要

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A zymogen of alkaline protease was isolated from cell-free extract of the Aspergillus strain W-1. After investigations on some properties of the zymogen and its activating factors, the following results were obtained.1) Zymogen of alkaline protease was salted out from cell-free extract of mycelia with 0.5〜0.8 saturation of (NH_4)_2SO_4.2) No activation of the zymogen was observed in alkaline media of above pH 7.4.3) The zymogen was markedly activated with the media of pH between 5 to 6,and full activation was attained after 60 min. at 40℃.4) The mode of activation of this zymogen was found to be different from that of various animals and of bacteria in several points ; first, the activation was not autocatalytic ; second, trypsin revealed no activation ; third, participation of any other activating enzymes was never considered.5) The activation was observed to be attributable to ionic strength and pH value of between 5 to 6 for its optimum range.6) By zone electrophoresis in veronal buffer of pH 8.6,zymogen migrated towards cathode, whild the active protease liberated from zymogen was found to migrate towards anode.
社団法人日本生物工学会の論文
1960-08-15