硫黄細菌の生化学的研究 : (第2報)菌の生育条件について

概要

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Some investigations have been reported concerning the physiology of T. thioxidans using cultures grown under static conditions, but little study has been carried out on cultures grown under conditions of mechanical shaking.Here, the authors investigated the physiology of T. thiooxidans using a culture under the latter conditions to obtain high yields of the cell.The findings are summarized as follows.1) The growth rate was less in the static culture than with shaking. It was considered that O_2 probably effected the growth as a limitting factor in static cultures.2) The experiments were conducted with shaking cultures with additions of various solid carbonates to the growing cultures in order to keep the pH 3.5. For that purpose, the best compound of these carbonates was found to be sodium salt followed by ammonium salt, after which came the calcium and barium carbonates. Barium salt was especially injurious on the growth.3) Ammonium ion was a very convenient source of nitrogen, but ammonium nitrate partially inhibited the growth.4) There was little difference of the growth rate among the cultures containing 0.1,0.2 or 0.5 per cent of ammonium sulfate. The concentration of 0.02 per cent of ammonium sulfate was a limiting factor in the growth.5) The influenee of various sulfur concentrations upon the sulfur oxidation was studies with a "sealed shaking culture".
社団法人日本生物工学会の論文
1964-12-25