麹菌のプロテアーゼの生成について (第1報) : Cell-free extractによるプロテアーゼの生成

概要

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An advanced culture medium revealing acceleration of the formation of protease by static culture of the Aspergillus strain W-1,was proposed. This medium is composed of glucose, peptone, KH_2PO_4,KCI and MgSO_4.With cell-free extract of mycelia harvested from the above medium, the following phenomena were observed.1) The activity of protease of the cell-free extract was found to be very low when it was newly prepared, but its activity increased gradually during incubation.2) The most active protease preparation of cell-free extract was obtained from the mycelia cultivated for 50〜60 hrs, and its formation was not affected by several inhibitors preventing protein synthesis.3) The maximum activity of protease of cell-free extract was observed when the extract was incubated for 3 hrs at 40℃ or 50 hrs at 0℃, and the amount of active protease produced from cell-free extract attains to a same extent irrespective of the incubating conditions.4) These facts suggest that active protease would exist in the mycelia as an inactive precursor or zymogen form, and de novo synthesis of the enzyme could be improbable.5) Ortho-phosphate remarkably accelerated hte liberation af active protease from zymogen. This effect of phosphate ion was intensified with its concentration up to 10^<-2>M.
社団法人日本生物工学会の論文
1960-08-15