Regulation of Bradykinin-Stimulated Cation Entry Into Endothelial Cells by tyrosine Kinase

元データ 1997-11-20 社団法人日本循環器学会

概要

In endothelial cells, bradykinin stimulates the release of intracellular Ca^<2+>, which is followed by the entry of extracellular Ca^<2+> into the cells. However, the mechanism underlying this Ca^<2+> entry is not well understood. To investigate the possible implication of tyrosine kinases in bradykinin-mediated Ca^<2+> signaling in endothelial cells, cultured porcine aortic endothelial cells were loaded with fura-2/AM, and Mn^<2+> influx into the cells was determined by the quenching of fluorescence intensity of fura-2 at 360 nm excitation. The tyrosine kinase inhibitors genistein and herbimycin A attenuated not only Ca^<2+> influx but also Mn^<2+> influx from the extracellular space without affecting the release of Ca^<2+> from internal stores in bradykinin-treated cells. In contrast to tyrosine kinase inhibitors, the tyrosine phosphatase inhibitor vanadate stimulated Ca^<2+> influx as well as Mn^<2+> influx. On the other hand, both an inactive analog of genistein, daidzein, and an inhibitor of diacylglycerol (DAG) kinase, ethylene glycol dioctanoate, were without effect on [Ca^<2+>]_i following the stimulation of agonist. These findings suggest that tyrosine kinase is involved in the regulation of cation influx in endothelial cells.

著者

Watanabe Hiroshi Department of Cardiology, Niigata City General Hospital
Ohno Ryuzo Department Of Internal Medicine Iii Hamamatsu University School Of Medicine
Takahashi Reiko Department of Internal Medicine III, Hamamatsu University School of Medicine
Zhang Xu-Xia Department of Internal Medicine III, Hamamatsu University School of Medicine
Hayashi Hideharu Photon Medical Research Center, Hamamatsu University School of Medicine
Kakizawa Hiroyasu Department of Internal Medicine III, Hamamatsu University School of Medicine
Hayashi Hideharu Division Of Cardiology Internal Medicine Iii Hamamatsu University School Of Medicine
Zhang Xu-xia Department Of Internal Medicine Iii Hamamatsu University School Of Medicine
Hayashi Hideharu Photon Medical Research Center Hamamatsu University School Of Medicine
Kakizawa Hiroyasu Department Of Internal Medicine Iii Hamamatsu University School Of Medicine
Ohno Ryuzo Department Of Built Environment Tokyo Institute Of Technology
Takahashi Reiko Department Of Clinical Pharmacology & Therapeutics Hamamatsu Unuversity School Of Medicine
Takahashi Reiko Department Of Anesthesia Ishikawa Prefectural Central Hospital
Watanabe Hiroshi Department Of Clinical Pharmacology & Therapeutics Hamamatsu University School Of Medicine
Watanabe Hiroshi Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
OHNO Ryuzo Department of Architecture, Kobe University

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