マウス試験用の麻ひ性貝毒抽出法の改善〔英文〕

概要

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Attempts were made to find the best conditions for extracting paralytic shellfish poison (PSP) from scallop digestive gland, the conditions in which low-toxic components with a carbamoyl-N-sulfo group are evaluated as high-toxic components. To a digestive gland homogenate was added an equal volume of various concentrations of HCl, HNO3 or H2SO4, and the mixtures were heated in boiling water. PSP toxicity, as analyzed by the mouse bioassay, increased with an increasing acid concentration of each extractant up to 1 N. The highest toxicity values of the extracts were 300MU/ml with 1 N HCl, 320MU/ml with 1 N HNO3, and 270MU/ml with 1 N H2SO4. A prolonged extraction (>30min) of the digestive gland with 1 N HCl resulted in a gradual decrease of toxicity, due to a partial degradation of PSP. Based on these results, it was proposed that to a digestive gland homogenate is added an equal volume of 1 N HCl and heated in boiling water for 5min, and the supernatant is used for the mouse bioassay. This extraction method is also applicable to other toxic shellfish.
日本水産學會の論文