ショウサイフグ肝臓高分子画分中におけるフグ毒の存在〔英文〕

概要

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High molecular weight substances associated with tetrodotoxin (TTX), which contained 82.2% of protein, were obtained from the extract of toxic liver of pufferfish Takifugu vermicularis snyderi by gel filtration on Sephacryl S-400 HR. The high molecular weight substances were hydrolyzable either with protease at 37°C, pH7.4, or with 0.5M HCl in boiling water, but neither with ribonuclease T2 nor with deoxyribonuclease I. Although the high molecular weight substances were nontoxic, their acid hydrolysates exhibited slight toxicity in mice when injected intraperitoneally. GC-MS analyses of the alkaline degradation products prepared from the protease digests revealed the presence of C9-base (2-amino-6-hydroxymethyl-8-hydroxy-quinazoline), indicating that the high molecular weight substances from the toxic liver contained TTX and/or its related substances. These results suggest that toxic pufferfish may partly accumulate TTX in liver, probably as a proteinic complex.
日本水産學會の論文