ヨロイイソギンチャクから単離した致死性溶血因子の物理化学的性質〔英文〕

概要

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Hemolysins were purified from whole bodies of the sea anemone Anthopleura japonica by acetone precipitation, ion-exchange chromatography on CM-cellulose, gel filtration on Sephadex G-75 and chromatofocusing. Two hemolysins (AJH-1 and 2) were detected and only AJH-2 was obtained in pure form as judged by analytical disc electrophoresis. AJH-2 exhibited a lethal activity in mice (LD30 78μg/kg, i, v. injection) as well as a hemolytic activity (106, 500 HU/mg against sheep erythrocytes). It was stable over a wide range of pH values from 2 to 11 but rather unstable to heat treatment. The molecular weight was determined to be 17, 800 by sedimentation equilibrium or 19, 500 by SDS-polyacrylamide gel electrophoresis, indicating that AJH-2 has no subunit structure. AJH-2 was a slightly basic protein (pI 8.0) and its amino acid composition was characterized by the absence of half-cystine. It was notable that these properties of AJH-2 were closely similar to those of parasitoxin, the major lethal hemolysin isolated from the sea anemone Parasicyonis actinostoloides.
日本水産學會の論文