フグ毒産生細菌の細胞内フグ毒産生に及ぼす培養条件の影響

概要

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The effect of various culture conditions on tetrodotoxins formation was studied using a strain F-9 isolated from the puffer fish Takifugu rubripes. The strain was identified as that similar to Vibrio nereis. The TTXs formation by the strain was proved by GC/MS analysis and the major toxin was identified as 4-epi TTX by HPLC. ORI medium gave a high toxin formation. Cells in the stationary phase showed a high toxicity. As compared with static culture, shaking culture was suitable for high toxin formation. Optimum temperatures for both growth and toxin formation were 27.5°C, but optimum NaCl concentrations for growth and toxin formation were 3 and 5%, respectively. These results suggested that various culture conditions influenced the productive ability of tetrodotoxins in the strain.
日本水産學會の論文