ナマズの膵臓エラスタ-ゼの特性〔英文〕

概要

論文の詳細を見る
An elastolytic enzyme, designated as elastase B, from the pancreas of the catfish Parasilurus asotus was characterized and compared with the well-established elastase from the porcine pancreas. The approximate molecular weight of catfish elastase B was estimated to be 26, 000 by SDS-polyacrylamide gel electrophoresis. The amino acid composition of catfish elastase B was similar to that of porcine elastase. Elastase B had high activities toward elastin, casein, hemoglobin, and Suc-(Ala)5 -NA, but very low activities toward Tos-Arg-OMe and Bz-Tyr-OEt. Catfish elastase B was strongly inhibited by DFP, PMSF, elastatinal, soybean trypsin inhibitor (Kunitz), and chicken ovoinhibitor. These findings indicate that catfish elastase B belongs to the group of serine proteinases and is closely related to porcine elastase.
日本水産學會の論文