魚類の銅代謝に関する研究-2-コイ組織における銅の蓄積部位

概要

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The site of copper accumulation was investigated in carp exposed to 0.1ppm copper solution for 2 weeks. Ceruloplasmin and direct-reacting copper contents in the serum of copper-exposed carp significantly increased as compared to those of normal carp. By copper loading, a statistically apparent increase of copper content was observed in the hepatopancreas, gills, intestine and kidney. Among these tissues, the highest copper content was found in the hepatopancreas. In the hepatopancreas, more than 70% of the copper was found in the supernatant fraction. Litlle, if any, copper was found in the ultrafiltrate from the supernatant treated with DIAFLO UM-2 membrane. Sephadex G-75 gel filtration analysis of the supernatant fraction demonstrated that the most part of copper accumulated in the hepatopancreas was bound to the relatively lower molecular weight protein.
日本水産學會の論文