アスコルビン酸欠乏ニジマスにおけるヒドロキシプロリン含量の低い"異常コラ-ゲン"の蓄積〔水産動物のL-アスコルビン酸に関する生化学的研究-15-〕

概要

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Amino acid compositions of collagens from the skin and vertebrae were determined in rainbow trout which had been fed with purified diets containing different amounts of L-ascorbic acid (AsA) for 11 weeks. Hydroxyproline contents of collagens from the skin and vertebrae were comparatively low in the groups receiving 0 or 2mg of AsA per 100g of diet, when compared with the control fish receiving 200mg of AsA per 100g of diet. The dietary AsA levels had little effect on the hydroxylysine content of collagen. The thermal stability of the skin collagen was investigated using changes in viscosity. The viscosity of collagen solution was measured after keeping the sample for 30min at some fixed temperature between 5 and 25°C. The denaturation temperatures of collagens from the groups receiving 5 to 200mg of AsA per 100 g of diet were 18.1 to 18.3°C, whereas those of collagens from the groups receiving 0 and 2mg of AsA per 100g of diet were 16.6 and 17.1°C, respectively. The viscosities of collagens from the groups receiving 5 to 200mg of AsA per 100g of diet were not decreased markedly up to 15°C, whereas those of collagens from the groups receiving 0 and 2mg of AsA per 100g of diet were low around 10°C. These results indicate that an underhydroxylated thermally labile collagen is formed and accumulates in the tissues of the fish which are fed with an AsA-deficient diet.
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