水産動物のL-アスコルビン酸に関する生化学的研究-VII : 魚類におけるデヒドロ-L-アスコルビン酸の還元

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The reduction of dehydro-L-ascorbic acid (DAsA) to L-ascorbic acid (AsA) was investigated in carp by means of radioactive tracer techniques. Six hours after intraperitoneal administration of DAsA-1-14C, 9.9% was recovered as“total AsA-14C”of which 96% was in the reduced form in the hepatopancreas. The reduction of DAsA-1-14C was also observed in other tissues such as kidney, eye, brain and blood. The reduction of DAsA was assayed in vitro by using the crude extract of carp hepatopancreas. The reduction of DAsA by GSH increased remarkably in the presence of crude extract as compared to nonenzymatic reduction. Heating the extract for 5 min at 100°C resulted in the complete loss of its activity. These results indicate the presence of DAsA-reductase in carp hepatopancreas. The enzyme in carp was found mainly in the hepatopancreas and kidney, and showed the same level of activity as the rat liver enzyme. The enzyme activity was also detected in all of the fishes so far tested.
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