水産動物のL-アスコルビン酸に関する生化学的研究-VI : コイの肝膵臓中のL-グロノラクトン脱水素酵素活性におよぼすα-トコフロール投与の効果

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In a previous paper4), it was shown that carps are able to synthesize L-ascorbic acid from D-glucose. The present paper deals with the effect of vitamin E on the biosynthesis of L-ascorbic acid in carps. After the administration of α-tocopherol into carps, the activity of L-gulonolactone dehydrogenase in hepatopancreas and the TBA*** value of viscera were determined. The results (Table 3) indicate that the enzyme activity in the hepatopancreas of carps administrated with α-tocopherol was higher than that of the untreated carps. The TBA value of viscera was also lower in the carps administrated with α-tocopherol than that of the untreated carps (Table 2). Moreover, the enzyme in hepatopancreas homogenate from the untreated carps was maintained stable by the addition of α-tocopherol (Fig. 1). The TBA value of the homogenate was also prevented from increasing by the addition of α-tocopherol (Fig. 2). From these results, it is assumed that the administration of vitamin E stimulates the biosynthesis of L-ascorbic acid as a result of its protecting action from the peroxide forma-tion of unsaturated fatty acids in the fish tissues.
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