魚類の銅代謝に関する研究-III : コイ肝膵臓におけるメタロチオネイン様タンパク質の存在

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The present study was undertaken to determine the appearance of copper-bound metallothionein in the hepatopancreas of carp kept in an 0.1 ppm copper solution for 2 weeks. The hepatic soluble fractions from control and copper-exposed fish were gel-filtrated on a Sephadex G-75 column, and the following results were obtained. 1) Copper as well as zinc was eluted in two fractions and most of the copper appeared in the second fraction (F-2) in the control. 2) Copper and free SH-group contents in F-2 from copper-exposed fish significantly increased as compared to those from control fish. 3) By incubation of the soluble fraction with p-chloro-mercuribenzoic acid it was shown that copper, zinc and free SH-groups were diminished in F-2 from copper-exposed fish. 4) When the soluble fractions from control and copper-exposed fish were incubated with copper, incorporation of the additive copper into F-2 was much higher in the copper-exposed fish than the control. In addition, the zinc in F-2 was replaced by copper and the free SH-groups in this fraction disappeared. 5) The molecular weight of F-2 was estimated to be about 10, 000 by gel-filtration on Sephadex G-75. These findings indicate that, in carp hepatopancreas, copper induces the synthesis of a copper-binding protein which can be identified as a metallothionein-like protein.
公益社団法人日本水産学会の論文