アールスメロンの純系および雑種系統の種子由来カルスにおける不定胚形成効率の比較

概要

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The efficiency of somatic embryogenesis in calli derived from mature seeds was compared with 6 pure lines and 3 hybrid lines of melon (Cucumis melo L. cv. Earls Favourite). Each of 25 seeds of these melon lines was cut into segments and separately cultured on a rotary shaker in a liquid MS medium containing 4μg/ml 2, 4-D and 0.1μg/ml BAP. Calli were induced at the cut-surface of seeds 3-4 days after incubation, and globular-stage embryos were produced in callus tissues of all melon lines used after 3 weeks of incubation. The highest production of the embryos was observed in the pure line, Fuyu 4. The embryo production was considerably higher in the hybrid line between Fuyu 4 and Natu 1, compared with those of respective pure lines. These somatic embryos were successfully regenerated into intact plants 30 days after transfer to a hormone-free MS medium.