トマト根に同時接種したフザリウム病菌の遺伝子標識法による識別

概要

論文の詳細を見る
記事区分：原著In our attempt to visualize the infection behavior of fungal wilt pathogens inoculated onto plant roots, the fungi were genetically marked with two reporter genes. Fusarium oxysporum f. sp. Melonis (FOM) and F. o. f. sp. Lycopersici (FOL) were transformed using the green fluorescence protein gene (GFP) and the β-glucuronidase gene (GUS), respectively. In the present study, we attempted to directly distinguish different formae speciales of F. oxysporum on the same plant roots by expression of different marker genes. Microconidia of GFP-marked FOM (FOM-001) and GUS-marked FOL (FOL-005) were inoculated onto roots of cotyledonal seedings, and inoculated roots were first observed under a fluorescence microscope in order to detect FOM-001 and then stained with X-gluc (substrate for GUS assay) in order to detect FOL-005 under a light microscope. Consequently, both transformed pathogens could be clearly distinguished at the same site of inoculation. These results suggest that the dual transformation of F. oxysporum is useful for analyzing the behavior of nonpathogenic F. oxysporum challenge-inoculated with pathogenic F. oxysporum.
2003-03-31