バラ葉外植片からのカルス誘導と個体再生系の確立

概要

論文の詳細を見る
Meristem tissues were excised from axillary buds of rose plants (Rosa hybrida cv. Carl Red) and cultured on media containing various concentrations of BA in order to clarify the condition for micro-propagation. The meristem tissues showed rapid and effective shoot formation when cultured with 1.0μg/ml BA. After 1 month of incubation, small leaflets of shoots were harvested and cultured on MS medium cotaining either IAA and BA or NAA and BA for callus induction. Adventitious buds were frequently induced in callus tissues cultured in the presence of 0.25μg/ml NAA and 0.005μg/ml BA, and effectively differentiated into shoots when transferred to MS medim containing 1.0μg/ml BA. Abundant roots were formed when shoots were cultured on 1/4×strength regeneration medium.
日本植物細胞分子生物学会の論文