トマト葉外植片カルスからのフザリン酸抵抗性再生体の選抜

概要

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Fusaric acid-resistant regenerants were selected in vitro by using the tissue culture system of tomato. The callus tissues were induced from the leaf explants of tomato and subcultured in a MS (0.01mg/l IAA and 0.1mg/l BAP) medium. After incubation for two weeks, a lot of green spots were produced in the callus tissues. The tissues were cut into small callus clumps so as to contain one green spot and transferred to a MS medium (0.1mg/l IAA and 1.0mg/l BAP) containing 50μg/ml fusaric acid. Fusaric acid-resistant regenerants were effectively developed from enlarged green spots and rooted in a hormone-free MS medium. For examining the resistance in regenerants at one month after transplantation to soil, their upper branches were cut and dipped in the solution containing 300μg/ml fusaric acid. In the branches of regenerants grown in the absence of fusaric acid, the resistance was fully maintained and expressed.
日本植物細胞分子生物学会の論文