In vitro growth and rapid multiplication of Lilium japonicum Thunb.

概要

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The seeds, embryos and shoot tips of Lilium japonicum Thunb. (which was collected in September) were used as explant sources for primary culture. Aseptic seedlings were not feasible because of low germination percentage. A sixty-one percent of embryos formed bulblets in the medium free from growth regulators. The enlargement of bulblets was closely related to the elongation of roots. Shoot tips enlarged and formed two or three bulblets on the medium containing NAA. The formation of bulblets was prevented by BAP. Shoot tips cultured in the medium with BAP formed watery calli, masses of shoot meristems (MSM) or protuberance tissues. The MSM was distinct from a callus because the former has the epidermis, outer layer and inner layer. The outer layer of MSM differentiated many shoot buds randomly. The volume of MSM increased gradually during its repetitive subculturing on the medium containing 10-5M of BAP. The protuberance tissue was a cormoid which was made of a enlarged base plate. Rapid multiplication was accomplished through the bulb scale culture under the conditions free from NAA and BAP and the bulblet regeneration from the MSM on the medium containing 10-6M of NAA.
日本植物細胞分子生物学会の論文