Study of subpopulations of infiltrating cells in the liver with experimentally induced hepatitis in mice.

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This study has been performed based on our model of experimental hepatitis prodused by immunizing C56BL/6 (B6) mice with syngeneic liver proteins and Freund's complete adjuvant. Peripheral blood lymphocytes (PBL), spleen cells and infiltrating cells in liver were obtained from hepatitis mice, and they were subjected to T-cell subpopulation analysis using specific antibodies; the reactivity of these cells to LSP was also studied by the lymphocyte transformation test. In this study, PBL and the T-cell fraction of spleen cells of hepatitis mice showed a high reactivity to syngeneic LSP. The population of T-cells checked by anti-Thy 1, 2 antibody was 32.7% in infiltrating cells in the liver of hepatitis mice and about 40% of this infiltrating T-cells were Ly 23 positive T-cell subpopulation (suppressor/cytotoxic). Ly 23 positive T-cell subpopulation was increased in the spleen of hepatitis mice compared to normal mice.<BR>By indirect immunofluorescent and immunoperoxidase study, Ly 23 positive T-cells were found around the periportal area and in the parenchyma of hepatitis mice.
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