Cytotoxicity of sensitized spleen cells from C57BL/6 mice with experimentally induced autoimmune hepatitis against normal syngeneic hepatocytes. Study of cell-mediated cytotoxicity by 51Cr release method.

概要

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To clarify the immune mechanisms responsible for the induction of experimental autoimmune hepatitis in C57BL/6 (B6) mice, we established a syngeneic assay system for cell-mediated cytotoxicity in which <SUP>51</SUP>Cr-labelled isolated hepatocytes from normal B6 mouse and sensitized spleen cells from the hepatitis B6 mice were used as target cells and effector cells respectively. In this assay, the sensitized spleen cells from hepatitis mice showed significantly higher cytotoxic activities against target cells than those from control animals. These findings suggest that the sensitized spleen cells play an important role in the induction of autoimmune hepatitis in B6 mice. This assay system is very useful because the H-2 restriction can be disregarded in the study of cell-mediated cytotoxicity.
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