Structure of Water-insoluble Glucan Synthesized by β-Transglycosylase of Trichoderma longibrachiatum
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概要
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A water-insoluble glucan was synthesized by the β-transglycosylase of Trichoderma longibrachiatum in a reasonable yield from 1% cellopentaose as the substrate after 48hr incubation. The glucan could be completely solubilized by cellulase to give glucose, cellobiose and cellotriose, although the reaction time was about 9 times longer than that required for the complete solubilization of the higher cellodextrin (DP 14) synthesized by the β-transglucosylase of Sclerotinia libertiana. The glucan was separated into two main fractions according to the solubility in NaOH solution, the major soluble in 4N-NaOH and the minor insoluble in it. The structure of the major fraction was investigated by methylation analysis, and it was clearly shown to be a linear β-1, 4- glucan having an average degree of polymerization of 19. These results indicated that the β- transglycosylase of Trichoderma longibrachiatum had a strict specificity of forming β- 1, 4-glucosidic linkages and also had a capacity to elongate the linkage to a higher extent.
- 社団法人 日本農芸化学会の論文
著者
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Oi Susumu
Department of Biology, Faculty of Science, Osaka City University
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Oi Susumu
Department Of Biology Faculty Of Science Osaka City University
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Tanaka Toshio
Department Of Applied Physics Faculty Of Engineering Tokyo University
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Tanaka Toshio
Department of Applied Chemistry, Faculty of Engineering, Osaka Univeristy
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TANAKA Toshio
Department of Biology, Faculty of Science, Osaka City University
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