Synthesis of β-1, 4-Glucan by the β-Transglucosylase of Sclerotinia libertiana

概要

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The β-transglucosylase of Sclerotinia libertiana catalyzed the reaction transferring the non-reducing end glucose of cellotetraitol (G4-OH) to the non-reducing end glucose of G4-OH, and both G3-OH and G5-OH were produced in the reaction at first. A water-insoluble glucan was synthesized by the enzyme, accompanied by the production of soluble cellooligosaccharides such as cellobiose, cellotriose, cellopentaose, and cellohexaose in a prolonged reaction with 2% cellotetraose. The water-insoluble glucan was found to be a linear β-1, 4-glucan having an average degree of polymerization of 14 by enzymatic hydrolysis test, methylation, and X-ray analyses. From the results, the β-transglucosylase of Sclerotinia libertiana was defined as a new enzyme able to synthesize higher cellodextrin by a disproportionation reaction of lower molecular weight cellooligosaccharides.
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