Interaction of the β-Transglycosylase of Trichoderma longibrachiatum with Cellulose
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概要
- 論文の詳細を見る
The effects of cellulose on the production and stimulation of β-transglycosylase were studied. The β-transglycosylase of Trichoderma longibrachiatum was produced specifically in the presence of cellulose in Czapeck-Dox medium containing sucrose as a sole carbon source. The enzyme activity was stimulated by the addition of cellulose in the reaction mixture, where the transfer reaction product (a water-insoluble glucan) was apparently synthesized on the surface of the added cellulose fibers. The hyphal wall fraction of the fungus had the same stimulatory effect on β-transglycosylase as the cellulose fibers. A cellulose-like material in this fraction was found by partial acid hydrolysis and gas chromatography. Cellotriose was the smallest substrate effective for the synthesis of a water-insoluble glucan in the presence of cellulose by the β-transglycosylase, though a significant amount of glucan could not be synthesized without the addition of cellulose.
- 社団法人 日本農芸化学会の論文
著者
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Oi Susumu
Department Of Biology Faculty Of Science Osaka City University
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Tanaka Toshio
Department Of Applied Physics Faculty Of Engineering Tokyo University
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Tanaka Toshio
Department of Applied Chemistry, Faculty of Engineering, Osaka Univeristy
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TANAKA Toshio
Department of Biology, Faculty of Science, Osaka City University
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