Isobavachalcone, a Chalcone Constituent of Angelica keiskei, Induces Apoptosis in Neuroblastoma(Pharmacology)

スポンサーリンク

概要

• 論文の詳細を見る

• Six chalcones from Angelica keiskei KOIDZUMI (Ashitaba in Japanese) and two chalcones from Humulus lupulus L. (hop) were examined for their cytotoxicity in two human neuroblastoma cell lines (IMR-32 and NB-39) and normal cells (primary culture of rat cerebellar granule cells) by [3-(4, 5)-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay. All chalcones exhibited cytotoxicity against neuroblastoma cells, and two of them (isobavachalcone and xanthoangelol H) had no effect on normal cells even at high concentration (10^<-4>M) exposure. Typical morphologic features of apoptosis, including cell shrinkage, chromatin condensation, nuclear fragmentation and formation of apoptotic bodies, were observed in isobavachalcone-treated cells by Hoechst 33342 staining. Western blot analysis showed that isobavachalcone significantly reduced pro-caspase-3 and pro-caspase-9, and subsequently increased the level of cleaved caspase-3 and cleaved caspase-9 in both neuroblastoma cell lines. Moreover, Bax was markedly induced by isobavachalcone application. These results suggest that isobavachalcone induces apoptotic cell death in neuroblastoma via the mitochondrial pathway and has no cytotoxicity against normal cells. Therefore, isobavachalcone may be applicable as an efficacious and safe drug for the treatment of neuroblastoma.

• 社団法人日本薬学会の論文
• 2007-10-01

著者

• Tabata Keiichi

  日本大学 薬学部臨床医学ユニット

• Ito Yoshihisa

  Research Unit Of Pharmacology Department Of Clinical Pharmacy College Of Pharmacy Nihon University

• ARAKAWA Motoki

  Research Unit of Pharmacology, Department of Clinical Pharmacy, College of Pharmacy, Nihon Universit

• Kohno Hideki

  Department Of Orthopaedic Surgery Interdisciplinary Graduate School Of Medicine And Engineering Univ

• Kimura Y

  Coll. Of Pharmacy Nihon Univ.

• Arakawa Motoki

  Research Unit Of Pharmacology College Of Pharmacy Nihon University

• SUZUKI Takashi

  Research Institute for Scientific Measurements,Tohoku University

• TABATA Keiichi

  Research Unit of Clinical Medicine, College of Pharmacy, Nihon University

• KIMURA Yumiko

  Research Unit of Analytical Chemistry of Pharmaceuticals, College of Pharmacy, Nihon University

• NAGAI Hisashi

  Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University

• AKIHISA Toshihiro

  Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University

• NISHIMURA Reiko

  Research Unit of Clinical Medicine, College of Pharmacy, Nihon University

• SAKUMA Atsuko

  Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University

• Nagai Hisashi

  Department Of Electrical Engineering Kyoto University

• Sakuma Atsuko

  Department Of Materials And Applied Chemistry College Of Science And Technology Nihon University

• Kohno Hideki

  Department Of Applied Molecular Chemistry College Of Industrial Technology Nihon University

• Suzuki Takashi

  School Of Pharmacy Nihon Univ.

• Ito Yoshihisa

  Research Unit Of Pharmacology College Of Pharmacy Nihon University

• Nishimura Reiko

  Research Unit Of Clinical Medicine College Of Pharmacy Nihon University

• Yasukawa Ken

  Coll. Of Pharmacy Nihon Univ.

• Akihisa T

  College Of Pharmacy Nihon University

• Akihisa Toshihiro

  日本大学 理工学部

• Akihisa Toshihiro

  Department Of Materials And Applied Chemistry Collegeof Science And Technology Nihon University

• Akihisa Toshihiro

  College Of Science And Technology Nihon University

• Ito Yoshihisa

  Research Center For Earthquake Prediction Disaster Prevention Research Institute Kyoto University

• Manosroi Aranya

  Fac. Of Pharmacy Chiang Mai Univ.

• Tabata Keiichi

  School Of Pharmacy Nihon University

• Takase S

  College Of Science And Technology (funabashi Campus) Nihon University

• Suzuki Takashi

  Research Faculty Of Agriculture Hokkaido University

• ITO Yoshihisa

  Research Unit of Pharmacology, College of Pharmacy, Nihon University

• SUZUKI Takashi

  Research Unit of Clinical Medicine, College of Pharmacy, Nihon University:Department of Pediatrics and Child Health, Nihon University School of Medicine

• ARAKAWA Motoki

  Research Unit of Pharmacology, College of Pharmacy, Nihon University

関連論文

• 天然物由来抗酸化性化合物のマスト細胞活性化抑制の作用機序
• Albanol A from the Root Bark of Morus alba L. Induces Apoptotic Cell Death in HL60 Human Leukemia Cell Line
• 24OP1-4 神経芽腫に対するアシタバ由来成分xanthoangelolの酸化ストレスを介するアポトーシス誘導効果(ポスター NBL(基礎),第22回日本小児がん学会 第48回日本小児血液学会 第4回日本小児がん看護研究会 同時期開催)
• OP38-4 神経芽腫細胞培養株に対するレイシ由来トリテルペンの分化誘導効果について(ポスター 神経芽腫9,第21回日本小児がん学会 第47回日本小児血液学会 同時期開催)
• Inhibitory Effect of Some Oxygenated Stigmastane-Type Sterols on 12-O-Tetradecanoylphorbol-13-acetate-Induced Inflammation in Mice
• Structures of Five Hydroxylated Sterols from the Seeds of Trichosanthes kirilowii MAXIM.
• Five D : C-Friedo-Oleanane Triterpenes from the Seeds of Trichosanthes kirilowii MAXIM. and Their Anti-inflammatory Effects
• Inhibitory Effect of Karounidiol on 12-O-Tetradecanoylphorbol-13-acetate-Induced Tumor Promotion
• クスノキ科植物のステロール成分
• EVS1-1 IgEによる未成熟ヒトマスト細胞のFcεRIを介する脱顆粒能の獲得とヒト型抗IgE抗体による抑制(EVS1 重症喘息の治療戦略〜IgEをターゲットとした治療,イブニングシンポジウム,第60回日本アレルギー学会秋季学術大会)
• Role of Caspase-12 in Amyloid β-Peptide-Induced Toxicity in Organotypic Hippocampal Slices Cultured for Long Periods
• S5-5 Potentiation of NMDA agonist-induced neuronal death by β-amyloid in rat organotypic hippocampal slice cultures : evidence for multiple cell death pathways(SYMPOSIUM 5: PHYSIOLOGY AND DISEASE OF GLUTAMATE IN NEURONAL AND GLIAL CELLS)(Proceedings of th
• N-Acetylcysteine and ebselen but not nifedipine protected cerebellar granule neurons against 4-hydroxynonenal-induced neuronal death
• N-acetylcysteine selectively protects cerebellar granule cells from 4-hydroxynonenal-induced cell death
• Modulation of Voltage-Gated Ca^ Current by 4-Hydroxynonenal in Dentate Granule Cells(Highlighted paper setected by Editor-in-cfiief)(Pharmacology)
• Protective effect of S-allyl-L-cysteine, a garlic compound, on amyloid β-protein-induced cell death in nerve growth factor-differentiated PC12 cells
• Morphine augments excitatory synaptic transmission in the dentate gyrus through GABAergic disinhibition
• Comparative study of survival signal withdrawal- and 4-hydroxynonenal-induced cell death in cerebellar granule cells
• The Melanogenesis-Inhibitory, Anti-Inflammatory, and Chemopreventive Effects of Limonoids in n-Hexane Extract of Azadirachta indica A. Juss. (Neem) Seeds
• 4-Hydroxyderricin from Angelica keiskei Roots Induces Caspase-dependent Apoptotic Cell Death in HL60 Human Leukemia Cells
• Survivin as a Prognostic Factor for Osteosarcoma Patients
• Elastic Soft Mode and Crystalline Field Effect of Kondo Lattice Substance;CeCu_6
• 168 天然物由来抗酸化性化合物によるマスト細胞活性化の修飾
• Cytotoxic activity of Perilla frutescens var. japonica leaf extract is due to high concentrations of oleanolic and ursolic acids
• Proteomic Analysis of Apoptosis Induced by Xanthoangelol, a Major Constituent of Angelica keiskei, in Neuroblastoma(Pharmacology)
• Isobavachalcone, a Chalcone Constituent of Angelica keiskei, Induces Apoptosis in Neuroblastoma(Pharmacology)
• A Novel Cyclophane Compound, CPPy, Facilitates NGF-Induced TrkA Signal Transduction and Induces Cell Differentiation in Neuroblastoma(Molecular and Cell Biology)
• Cancer Chemopreventive Effects and Cytotoxic Activities of the Triterpene Acids from the Resin of Boswellia carteri(Pharmacognosy)
• Xanthoangelol, a Major Chalcone Constituent of Angelica keiskei, Induces Apoptosis in Neuroblastoma and Leukemia Cells(Pharmacology)
• Triterpene Alcohols and Sterols from the Fern Gleichenia japonica SPR.
• Activation of Dopamine D4 Receptors Is Protective Against Hypoxia/Reoxygenation-Induced Cell Death in HT22 Cells
• Development of acute lymphoblastic leukemia following hemophagocytic lymphohistiocytosis : is it secondary leukemia ?
• 24OP1-11 神経芽腫細胞培養株に対するアシタバ由来成分isobavachalconeの選択的アポトーシス誘導効果について(ポスター NBL(基礎),第22回日本小児がん学会 第48回日本小児血液学会 第4回日本小児がん看護研究会 同時期開催)
• Acoustic de Haas-van Alphen Effect of LaB_6
• 6S, 8R-Stereochemistry of the C_- and C_-Alkane-6,8-diols Isolated from Three Compositae Flowers
• Observation of Acoustic de Haas-van Alphen Effect in Heavy Electron Compound CeB_6
• Heat Capacity of (C_nH_NH_3)_2FeCl_4; n=1 and n=2
• Structural Phase Transition of Layer Compound (C_2H_5NH_3)_2FeCl_4
• 24OP1-19 神経芽腫細胞に対する乳香由来トリテルペンの抗腫瘍効果(ポスター NBL(基礎),第22回日本小児がん学会 第48回日本小児血液学会 第4回日本小児がん看護研究会 同時期開催)
• Useful Markers for Detecting Minimal Residual Disease in Cases of Neuroblastoma(Molecular and Cell Biology)
• Treatment of Ewing's Sarcoma Using an Antisense Oligodeoxynucleotide to Regulate the Cell Cycle(Molecular and Cell Biology)
• Analyses of Novel Prognostic Factors in Neuroblastoma Patients(Molecular and Cell Biology)
• Significance of Survivin mRNA Expression in Prognosis of Neuroblastoma(Analytical Biochemistry)
• Usefulness of Tyrosine Hydroxylase mRNA for Diagnosis and Detection of Minimal Residual Disease in Neuroblastoma (Biochemistry/Molecular Biology)
• Diagnostic Significance and Clinical Applications of Chimeric Genes in Ewing's Sarcoma
• Meniscal ganglion cysts without accompanying meniscal tears : a report of 2 cases
• Mobile submuscular mass resembling a hard-boiled egg : case report
• Clusia guaviarensisからの新規トリテルペノイド(17α,20R)-Dammara-12,24-dien-3β-ol
• Detection of Chimeric Genes in Ewing's Sarcoma and Its Clinical Applications
• Magneto-Acoustic Effect of CeCu_6 at Low Temperatures
• Structural Phase Transition and Acoustic de Haas-van Alphen Effect of SmCu_6
• On the Structural Phase Transitions in (C_2H_5NH_3)_2FeCl_4.I.Brillouin Scattering
• メグスリノキ樹皮およびヤマモモ樹皮由来環状ジアリールヘプタノイドのメラニン産生抑制効果 (特集 最新のメラニン研究と美白剤の開発)
• 天然トリテルペンの抗炎症,抗腫瘍および発がん予防機能 (特集 脂質と脂溶性化合物の健康栄養機能--最近の研究の進歩)
• JAWC 2000 に参加して
• 5-Dehydrokarounidiol [D : C-Friedo-oleana-5,7,9(11)-triene-3α, 29-diol], a Novel Triterpene from Trichosanthes kirilowii MAXIM.
• 7-Oxodihydrokarounidiol [7-Oxo-D : C-friedo-olean-8-ene-3α, 29-diol], a Novel Triterpene from Trichosanthes kirilowii
• Melanogenesis Inhibitory and Free Radical Scavenging Activities of Diarylheptanoids and Other Phenolic Compounds from the Bark of Acer nikoense(Pharmacognosy)
• Anti-inflammatory and Antitumor-Promoting Effects of the Triterpene Acids from the Leaves of Eriobotrya japonica(Pharmacognosy)
• Triterpene Acids from the Leaves of Perilla frutescens and Their Anti-inflammatory and Antitumor-promoting Effects
• Surface Morphology of YBa_2Cu_3O_ Films Prepared by Metalorganic Chemical Vapor Deposition Using Liquid Sources
• Preparation of A-axis Oriented YBa_2Cu_3O_ Films by Metalorganic Chemical Vapor Deposition Using Liquid Sources
• Preparation of YBa_2Cu_3O_ Thin Films by Metal-Organic Chemical Vapor Deposition Using Liquid Sources
• 3-O-(E)-p-Coumaroyl Tormentic Acid from Eriobotrya japonica Leaves Induces Caspase-Dependent Apoptotic Cell Death in Human Leukemia Cell Line
• Anti-Inflammatory and Chemopreventive Effects of Triterpene Cinnamates and Acetates from Shea Fat
• Inhibitory Effect of Cycloartenol Ferulate, a Component of Rice Bran, on Tumor Promotion in Two-Stage Carcinogenesis in Mouse Skin
• Triterpene Alcohols from Camellia and Sasanqua Oils and Their Anti-inflammatory Effects
• 4-Epicycloeucalenone and 4-Epicyclomusalenone : Two 3-Oxo-28-norcycloartanes from the Fruit Peel of Musa sapientum L.
• Cyclokirilodiol and Isocyclokirilodiol : Two Novel Cycloartanes from the Seeds of Trichosanthes kirilowii MAXIM.
• Inhibitory Effect of Di- and Trihydroxy Triterpenes from the Flowers of Compositae on 12-O-Tetradecanoylphorbol-13-acetate-Induced Inflammation in Mice
• Helianol [3,4-seco-19(10→9)abeo-8α, 9β, 10α-eupha-4,24-dien-3-ol], a Novel Triterpene Alcohol from the Tabular Flowers of Helianthus annuus L.
• Eight Novel Sterols from the Roots of Bryonia dioica JACQ.
• Inhibitory Effects of Sterols Isolated from Chlorella vulgaris on 12-O-Tetradecanoylphorbol-13-acetate-Induced Inflammation and Tumor Promotion in Mouse Skin
• A Novel Crosslinking-Decrosslinking System of Polymers Having Spiro Orthoester Moieties in the Side Chains
• 3-Epicabraleahydroxylactone and Other Triterpenoids from Camellia Oil and Their Inhibitory Effects on Epstein-Barr Virus Activation
• 天然甘味料の発がん予防効果
• Acerogenin M, a Cyclic Diarylheptanoid, and Other Phenolic Compounds from Acer nikoense and Their Anti-inflammatory and Anti-tumor-Promoting Effects
• Antitubercular Activity and Inhibitory Effect on Epstein-Barr Virus Activation of Sterols and Polyisoprenepolyols from an Edible Mushroom, Hypsizigus marmoreus(Pharmacognosy)
• Antitubercular Activity of Triterpenoids from Asteraceae Flowers(Pharmacognosy)
• Selinidin Suppresses IgE-Mediated Mast Cell Activation by Inhibiting Multiple Steps of FcεRI Signaling(Pharmacology)
• Triterpene Alcohol and Fatty Acid Composition of Shea Nuts from Seven African Countries
• Melanogenesis Inhibitory Activities of Iridoid-, Hemiterpene-, and Fatty Acid-glycosides from the Fruits of Morinda citrifolia (Noni)
• Melanogenesis Inhibitory, Anti-Inflammatory, and Chemopreventive Effects of Limonoids from the Seeds of Azadirachta indicia A. Juss. (Neem)
• Dehydrotrametenonic Acid and Dehydroeburiconic Acid from Poria cocos and Their Inhibitory Effects on Eukaryotic DNA Polymerase α and β
• Astertarone A : A Triterpenoid Ketone Isolated from the Roots of Aster tataricus L.
• Inhibitory Effect of Stevioside on Tumor Promotion by 12-O-Tetradecanoylphorbot-13-acetate in Two-Stage Carcinogenesis in Mouse Skin
• Modeling slip processes at the deeper part of the seismogenic zone using a constitutive law combining friction and flow laws
• On the Structural Phase Transitions in (C_2H_5NH_3)_2FeCl_4.II.Landau Theory
• Astertarone B, a Hydroxy-Triterpenoid Ketone from the Roots of Aster tataricus L.
• Cycloarta-16,24-dien-3β-ol : Revised Structure of Cimicifugenol, a Cycloartane Triterpenoid
• Influence of light direction on appearance of solid object
• Activation of Dopamine D_4 Receptors Is Protective Against Hypoxia/Reoxygenation-Induced Cell Death in HT22 Cells
• Chemiluminescent Enzyme Immunoassay for Measuring Leptin
• Induction of Apoptosis in Human Leukemia Cell (Jurkat) by Neolignans Isolated from Seeds of Licaria puchury-major
• Ferrearin C induces apoptosis via heme oxygenase-1 (HO-1) induction in neuroblastoma
• Efficient hydrogen production from acetate through isolated Rhodobacter sphaeroides(BIOCHEMICAL ENGINEERING)
• Vitamin K3 Analogs Induce Selective Tumor Cytotoxicity in Neuroblastoma
• Monoclonal antibodies against large oval aggregates of Aβ_(BIOMEDICAL ENGINEERING)
• The Inhibition of Human Tumor Cell Proliferation by RNase Pol, a Member of the RNase T1 Family, from Pleurotus ostreatus
• The Inhibition of Human Tumor Cell Proliferation by RNase Pol, a Member of the RNase T1 Family, from Pleurotus ostreatus

もっと見る 閉じる

スポンサーリンク