Significance of Survivin mRNA Expression in Prognosis of Neuroblastoma(Analytical Biochemistry)

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概要

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• Neuroblastoma (NB) is the most common malignant solid tumor in childhood, and among all childhood malignancies is second in prevalence only to leukemia. In NB we need to both make an accurate diagnosis and rapidly analyze the expression of genetic prognostic factors such as MYCN, H-ras, and trkA. Moreover, it has recently become important to analyze the expression of survivin mRNA, a member of the inhibitor of apoptosis protein family. Expression of the survivin gene is related to tumorigenesis and inhibition of apoptosis in some malignant tumors. We investigated its expression by reverse transcription-polymerase chain reaction (RT-PCR) in NB cell lines (SK-N-SH, NB-39, and IMR-32), two normal blood cell samples, and 13 clinical NB tumor samples. All three NB cell lines had high levels of mRNA expression for this gene, but normal blood cells had no expression. We detected expression of survivin mRNA in 7 of the 13 NB tumor samples (54%). Two NB patients were in stage I disease, 6 in stage II, and 5 in stage IV_A. Quantitative analysis by RT-PCR revealed that the ratio between survivin mRNA and human glyceraldehyde-3-phosphate dehydrogenase (h-GAPDH) mRNA was very low in stages I and II (0-0.017). In contrast, in advanced NBs (stage IV_A) the ratio was much higher (0-0.050). The prognoses of the three patients in the advanced stage who had high ratios of expression were poor. A high level of expression of survivin mRNA indicates a high grade of malignancy, high likelihood of recurrence, and poor prognosis.

• 公益社団法人日本薬学会の論文
• 2005-04-01

著者

• Nemoto Norimichi

  Department of Pathology, Nihon University School of Medicine

• MUGISHIMA Hideo

  Department of Division of Cell Regeneration and Transplantation, Nihon University School of Medicine

• Mugishima H

  Department Of Pediatrics Division Of Cell Regeneration And Transplantation Advanced Medical Research

• Mugishima Hideo

  日本大学 医学部小児科学

• Mugishima H

  日本大学 医学部小児科学

• CHIN MOTOAKI

  Department of Pediatrics, Advanced Medical Research Center, Nihon University School of Medicine

• Yoshida Yukihiro

  Department of Orthopaedic Surgery, Nihon University School of Medicine

• Suzuki T

  Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences:core Research Fo

• Ito R

  Medicinal Chemistry Research Laboratories Sankyo Co. Ltd.

• Ito Rie

  Clinical Pharmacy College Of Pharmacy Nihon University

• YAMAGUCHI Yusuke

  Clinical Pharmacy, College of Pharmacy, Nihon University

• ASAMI Satoru

  Clinical Pharmacy, College of Pharmacy, Nihon University

• SuzuKi Takashi

  Clinical Pharmacy, College of Pharmacy, Nihon University

• Nemoto Norimichi

  Division Of Pathology Department Of Pathology And Microbiology Nihon University School Of Medicine

• Nemoto Norimichi

  Department Of Iind Pathology Nihon University School Of Medicine

• Yoshida Y

  Department Of Orthopedic Surgery Nihon University

• Suzuki T

  Department Of Pharmaceutics College Of Pharmacy Nihon University

• Suzuki Takashi

  School Of Pharmacy Nihon Univ.

• Hinotsu Shiro

  Japanese Wilms Tumor Study (JWiTS) Group

• Chin Motoaki

  Japanese Wilms Tumor Study (JWiTS) Group

• Mugishima Hideo

  Japanese Wilms Tumor Study (JWiTS) Group

• SHICHINO Hiroyuki

  Department of Pediatrics, School of Medicine

• ASAMI Satoru

  Research Unit of Clinical Medicine, College of Pharmacy, Nihon University

• MOTOHASHI Shigeyasu

  Chemistry, College of Pharmacy, Nihon University

• OOTSUKA Susumu

  Clinical Pharmacy, College of Pharmacy, Nihon University

• Yoshida Yukihiro

  Department Of Information Systems Engineering Osaka University

• Motohashi Shigeyasu

  Chemistry College Of Pharmacy Nihon University

• Asami Satoru

  Research Unit Of Clinical Medicine College Of Pharmacy Nihon University

• Osaka S

  Department Of Orthopedic Surgery Nihon University Nerima Hikarigaoka Hospital

• Ootsuka Susumu

  Research Unit Of Clinical Medicine College Of Pharmacy Nihon University:department Of Hospital Pharm

• Suzuki Takashi

  Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences:core Research Fo

• Nemoto Norimichi

  Nihon Univ. School Of Medicine Tokyo Jpn

• Shichino Hiroyuki

  Department Of Pediatrics Division Of Cell Regeneration And Transplantation Advanced Medical Research

• Chin Motoaki

  Department Of Pediatrics Division Of Cell Regeneration And Transplantation Advanced Medical Research

• Yamaguchi Yusuke

  Clinical Pharmacy College Of Pharmacy Nihon University

• Yoshida Y

  Department Of Orthopedic Surgery Nihon University School Of Medicine

• Yoshida Yukihiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University

• Chin Motoaki

  Department Of Pediatrics And Child Health Nihon University School Of Medicine

• Takase S

  College Of Science And Technology (funabashi Campus) Nihon University

• Hinotsu Shiro

  Department Of Pharmacoepidemiology Kyoto University Graduate School Of Medicine And Public Health

• Suzuki Takashi

  Clinical And Biotechnology Business Unit Shimadzu Corporation

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