Statin Promotes Mineralization Potential in MC3T3-E1 Nonmineralizing Subclone
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概要
- 論文の詳細を見る
Statins, cholesterol-lowering drugs, have been recently reported to activate the promoter of the bone morphogenic protein-2 (BMP-2) gene, and stimulate bone formation. In this study, we investigated whether statins promote the mineralization of a subclonal cell line with low differentiation/mineralization potential derived from a murine pro-osteoblastic cell line, MC3T3-E1. Two subclonal cell lines, a nonmineralizing subclone (subclone N) and a mineralizing subclone (subclone M) after growth in the presence of ascorbic acid, were used in this study. Mineralization was detected by von Kossa staining 14 days after the addition of ascorbic acid with or without mevastatin. Total RNA was extracted from the cultured cells 3 days after stimulation, and the mRNA expression level of osteoblast-related molecules was measured using real-time quantitative RT-PCR. Only subclone M formed a mineralized matrix by stimulation of ascorbic acid alone. When mevastatin was added to the ascorbic acid-containing medium, not only subclone M but also subclone N formed a mineralized matrix. The mRNA expression levels of BMP-2 and osteocalcin in subclone N were significantly lower than those in subclone M. When mevastatin was added, both the mRNA levels in subclone N significantly increased, and there was no significant difference between the two subclones. These results demonstrate that statins promote mineralization in nonmineralizing osteoblasts through the induction of BMP-2 and osteocalcin. Hence, statins could be very useful for the promotion of osteoblast differentiation and mineralization in regenerative bone.
- 日本再生歯科医学会の論文
- 2006-03-30
著者
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Kawamoto Akiyo
Department of Geriatric Dentistry, Osaka Dental University
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Okazaki Joji
Department of Removable Prosthodontics and Occlusion, Osaka Dental University
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Komasa Yutaka
Department of Geriatric Dentistry, Osaka Dental University
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Tamura Isao
Department of Biochemistry Osaka Dental University
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Goda Seiji
Department of Biochemistry Osaka Dental University
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Ikeo Takashi
Department of Biochemistry Osaka Dental University
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Goda Seiji
大阪歯科大学 大学院歯学研究科口腔外科学
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Goda Seiji
Biochemistry Osaka Dental University
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Kawamoto Akiyo
Department Of Geriatric Dentistry Osaka Dental University
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Kamada Aiko
大阪歯科大学 生化学
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Ikeo Takashi
大阪歯科大学 生化学
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Morita Shosuke
First Department of Oral and Maxillofacial Surgery, Osaka Dental University
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HOKUGO Akishige
First Department of Oral and Maxillofacial Surgery, Osaka Dental University
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KAWADA Aiko
Department of Biochemistry, Osaka Dental University
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Morita Shosuke
First Department Of Oral And Maxillofacial Surgery Osaka Dental University
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Morita Shosuke
大阪歯科大学 大学院歯学研究科口腔外科学
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Hokugo Akishige
First Department Of Oral And Maxillofacial Surgery Osaka Dental University
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Kamada A
Department Of Geriatric Dentistry Osaka Dental University
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Morita Shosuke
Osaka Dental Univ. Osaka Jpn
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Kamada A
Department Of Biochemistry Osaka Dental University
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Okazaki J
Department Of Removable Prosthodontics And Occlusion Osaka Dental University
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Komasa Y
Graduate School Of Dentistry (geriatric Dentistry) Osaka Dental University
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Okazaki Joji
Department Of Geriatric Dentistry Osaka Dental University
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Ikeo T
Department Of Biochemistry Osaka Dental University
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Komasa Yutaka
Department Of Geriatric Dentistry Graduate School Of Geriatric Dentistry Osaka Dental University
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Komasa Yutaka
Department of Geriatric Dentistry , Osaka Dental University
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