Effect of hepatocyte growth factor on synthesis of extracellular matrix in human gingival fibroblasts

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Hepatocyte growth factor (HGF), a multifunctional cytokine produced by mesenchymal cells, is known to mediate the mesenchymal-epithelial interaction in a paracrine manner. Little is known about the direct effect of the factor on the function of mesenchymal cells. We investigated mRNA expression of type I collagen and decorin in human gingival fibro-blasts stimulated by HGF. Quantitative analysis using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) detection system demonstrated that HGF increased both the mRNA expression levels for type I collagen and for decorin in cultured gingival fibroblasts. The mRNA expression of decorin was increased significantly (p<0.05) 12h after HGF stimulation, and then that of type I collagen was markedly increased (p<0.01) at 24 h. These results suggest that HGF is likely to directly accelerate the extracellular matrix production by gingival fibroblasts. (J Osaka Dent Univ 2003; 37: 119-122.)
大阪歯科学会の論文