Expression of decorin mRNA and protein in human gingival fibroblasts induced by interleukin-1β
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概要
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Decorin, a small dermatan sulfate proteoglycan, is distributed throughout the extracellular matrix of periodontal tissues and is an important mediator of tissue development and repair. Previously, we demonstrated a significant increase in decorin mRNA expression in gingival tissue with chronic periodontal disease. Real-time reverse transcriptase-polymerase chain reaction (PCR) offers a rapid, sensitive method to quantify this substance. To elucidate the fluctuation of decorin mRNA during the process of inflammation,we examined its expression in cultured human gingival fibroblasts stimulated by inter-leukin (IL)-1β for 3 to 48 h using a real-time PCR detection system. Normal human periodontal fibroblasts were obtained from explant cultures of human gingiva. Total RNA was reverse-transcripted and amplified by real-time quantitative PCR to determine the mRNA level. TGF-β, type I collagen and IL-6 mRNA expression were also investigated. Decorin and collagen mRNA in fibroblasts stimulated by IL-1β was expressed weakly at 3 h, increased significantly at 8 and 24 h, and then gradually decreased at 48 h. TGF-β mRNA was also strongly expressed at 24 h. IL-6 mRNA levels apparently peak at 8 h, and were decreased at 24 and 48 h. There was a high correlation (r=0.8626) between decorin and TGF-β mRNA expression. These results indicate that gingival fibroblasts stimulated by IL-1β actively express IL-6 mRNA as an early event of inflammation, followed by decorin, collagen and TGF-β, which are associated with collagen fibrils, as a later event coexisting with destruction and repair.
- 大阪歯科学会の論文
著者
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Kawamoto Akiyo
Department of Geriatric Dentistry, Osaka Dental University
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Okazaki Joji
Department of Removable Prosthodontics and Occlusion, Osaka Dental University
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Komasa Yutaka
Department of Geriatric Dentistry, Osaka Dental University
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Kawamoto Akiyo
Department Of Geriatric Dentistry Osaka Dental University
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Kamada Aiko
大阪歯科大学 生化学
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Kawamoto Akiyo
Graduate School of Dentistry (Geriatric Dentistry), Osaka Dental University
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Kamada A
Department Of Geriatric Dentistry Osaka Dental University
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Kamada A
Department Of Biochemistry Osaka Dental University
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Okazaki J
Department Of Removable Prosthodontics And Occlusion Osaka Dental University
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Komasa Y
Graduate School Of Dentistry (geriatric Dentistry) Osaka Dental University
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Okazaki Joji
Department Of Geriatric Dentistry Osaka Dental University
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Komasa Yutaka
Department Of Geriatric Dentistry Graduate School Of Geriatric Dentistry Osaka Dental University
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Komasa Yutaka
Department of Geriatric Dentistry , Osaka Dental University
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