How changes in localized pressure under an experimental acrylic plate covering the palatal mucosa affect salivary cortisol concentration and α-amylase activity
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概要
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We quantified the degree of discomfort induced by plates of different thicknesses placed under an experimental acrylic plate (exp. plate) covering the palatal mucosa in an attempt to model ill-fitting dentures. Both salivary cortisol concentration and α-amylase activity were measured in response to the discomfort caused by varying localized pressure on the experimental acrylic plate. Nineteen healthy subjects with an average age of 25±2 years were included in this study. All subjects were seated and examined under the five experimental conditions of N (not wearing an exp. plate), D (wearing an exp. plate), DP 1 (wearing an exp. plate with a 0.1-mm-thick localized pressure plate), DP 2 (wearing an exp. plate with a 0.2-mm-thick localized pressure plate) and DP 3 (wearing an exp. plate with a 0.5-mm-thick localized pressure plate). Subjects were then asked to complete a Visual Analogue Scale (VAS) during each condition. Saliva samples were collected at 0, 5, 10, 15 and 20min after the start of each condition. Cortisol concentration and α-amylase activity were measured using enzyme-linked immunosorbent assay and kinetic analysis. Salivary cortisol concentration and α-amylase activity significantly increased as the pain and discomfort resulting from localized pressure increased. Cortisol concentration and α-amylase activity for condition D were significantly greater than that for N. Although the pain score, as measured by VAS, was the same for conditions N and D, significant differences in discomfort were found between N and D. We concluded that the severity of palatal mucosa stimulation and discomfort may be objectively assessed using salivary cortisol concentration and α-amylase activity.
- 大阪歯科学会の論文
著者
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Okazaki Joji
Department of Removable Prosthodontics and Occlusion, Osaka Dental University
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Ito Hidetaka
Graduate School of Dentistry (Removable Prosthodontics and Occlusion), Osaka Dental University
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Maeda Teruta
Department of Removable Prosthodontics and Occlusion, Osaka Dental University
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Ito Hidetaka
Graduate School Of Dentistry (removable Prosthodontics And Occlusion) Osaka Dental University
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Okazaki Joji
Department Of Removable Prosthodontics And Occlusion Osaka Dental University
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Maeda Teruta
Osaka Dental Univ. Hirakata‐shi Jpn
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Maeda Teruta
Department Of Removable Prosthodontics And Occlusion Osaka Dental University
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Okazaki Joji
Department Of Geriatric Dentistry Osaka Dental University
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Ito Hidetaka
Department Of Removable Prosthodontics And Occlusion Osaka Dental University
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Maeda Teruta
Department of Removable Prosthodontics and Occlusion, Osaka Dental University:Department of Interdisciplinary Dentistry for Postgraduate Trainees, Osaka Dental University
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