High-Performance Liquid Chromatography Analysis of Chondroitin Sulfate Isomers in Human Periodontium

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概要

• 論文の詳細を見る

• We investigated the chondroitin sulfate in human periodontal samples (gingiva, periodontal ligament, cementum and alveolar bone) collected for orthodontic reasons. Glycosaminoglycans (GAGs) were extracted from the periodontium by enzyme digestion, and unsaturated disaccharide isomers of chondroitin sulfate were obtained by chondroitinase AC II and hyaluronidase digestion. The isomers were analyzed by high-performance liquid chromatography. Chondroitin sulfate was found in all four types of periodontal tissue; its unsaturated disaccharide isomers consisted in ΔDi-0S, ΔDi-6S, ΔDi-4S, ΔDi-diS_E and ΔDi-triS. These four types of periodontal tissue showed different molar ratios of the unsaturated disaccharides. The ratio of ΔDi-4S to ΔDi-6S was greater in the calcified than in the uncalcified tissue.

• 大阪歯科学会の論文
• 1993-10-11

著者

• FUJITA Atsushi

  Department of Neurosurgery, Kobe University Graduate School of Medicine

• Kamada Aiko

  Department of Biochemistry Osaka Dental University

• Kawamoto Akiyo

  Department Of Geriatric Dentistry Osaka Dental University

• OKAZAKI Joji

  First Department of Prosthodontics, Osaka Dental University

• SAKAKI Tetsuya

  Department of Biochemistry, Osaka Dental University

• GONDA Yoshimichi

  First Department of Prosthodontics, Osaka Dental University

• Sakaki Toshio

  Second Department of Oral and Maxillofacial Surgery, Osaka Dental Universty

• Sakaki Tetsuya

  Department Of Biochemistry Osaka Dental University

• Sakaki Tetsuya

  大阪歯科大学 第2口腔外科

• Gonda Y

  Osaka Dental Univ. Osaka Jpn

• Gonda Yoshimichi

  大阪歯科大学 高齢者歯科

• Gonda Yoshimichi

  Department Of Geriatric Dentistry Osaka Dental University

• Sakaki T

  Second Department Of Oral And Maxillofacial Surgery Osaka Dental Universty

• Fujita A

  Department Of Biochemistry Osaka Dental University

• Sakaki Toshio

  Department Of Biochemistry Osaka Dental University

• Kamada A

  Department Of Biochemistry Osaka Dental University

• Okazaki J

  Department Of Removable Prosthodontics And Occlusion Osaka Dental University

• Okazaki Joji

  First Department Of Prosthodontics Osaka Dental University

• Fujita Atsushi

  Department Of Internal Medicine Osaka Medical College

• Okazaki Joji

  Department Of Geriatric Dentistry Osaka Dental University

• Fujita Atsushi

  Department Of Biochemistry Osaka Dental University

• Gonda Yoshimichi

  First Department Of Prosthodontics Osaka Dental University

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