Response of Collagen XII Splice Variants in Human Osteoblast-like Cells to IL-1β
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概要
- 論文の詳細を見る
Collagen XII, a fibril-associated collagen (FACIT), is characterized by a short triple-helical domain with three extended noncollagenous NC3 domains, and may contribute to the stability of collagen I fibrils. Differential splicing within the NC3 domain results in large and small variants of collagen XII. Little is known about the role of this FACIT collagen in bone metabolism. In this study, we demonstrated the gene expression of collagen XII splice variants in human osteoblast-like cells, and their responses to IL-1β as a bone resorptive agent. Western blot analysis also showed the protein expression of both long- and short-form splice variants, α1(XIIA) and α1(XIIB) respectively, in osteoblast-like cells. When cells were cultured for 48 h with IL-1β, mRNA expression levels of collagen I, osteocalcin and whole collagen XII decreased compared with controls (p<0.01). Interestingly, after IL-1β stimulation, the level of α1(XII) chain de-creased with time, although α1(XIIA) increased significantly by 2h, and then decreased to a lower level compared with non-stimulated controls. Our results clarify that the collagen XII gene is transcribed in human osteoblast-like cells, and indicate that the transcription of splice variants may be influenced by the bone resorptive agent.
- 日本再生歯科医学会の論文
- 2007-09-30
著者
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Kawamoto Akiyo
Department of Geriatric Dentistry, Osaka Dental University
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Kamada Aiko
Department of Biochemistry Osaka Dental University
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Ikeo Takashi
Department of Biochemistry Osaka Dental University
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Kawamoto Akiyo
Department Of Geriatric Dentistry Osaka Dental University
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Kamada Aiko
大阪歯科大学 生化学
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Ikeo Takashi
大阪歯科大学 生化学
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TANAKA Yoshihito
Department of Biochemistry, Osaka Dental University
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Kamada A
Department Of Geriatric Dentistry Osaka Dental University
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Tanaka Yoshihito
Department Of Biochemistry Osaka Dental University
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Kamada A
Department Of Biochemistry Osaka Dental University
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Ikeo T
Department Of Biochemistry Osaka Dental University
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