Expression of early growth response-1 and -2 in human periodontal ligament fibroblasts

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概要

• 論文の詳細を見る

• We investigated expression of early growth response (EGR)-1 and EGR-2 in human periodontal ligament fibroblasts (PLFs). After cultivating PLFs in Dulbecco's modified Eagle's medium (DME) for two days without 10% fetal calf serum (FCS), we incubated them in DME supplemented with FCS for 15, 30, 45, 60 and 120min. 5-bromo 2'-deoxyuridine incorporation showed a peak in the PLFs treated for 45 min. Expressions of EGR-1 protein and mRNA were seen beginning 15 min after treatment. EGR-1 protein gradually increased during this period. Anti-EGR-1 antibody strongly reacted with the nuclear region at 30 min. Maximum expression of EGR-2 protein was seen at 30 min, and it was localized in and around the nucleus. Expression of EGR-2 mRNA showed a peak at 15 min. These results demonstrate that EGR expression is more pronounced in FCS-stimulated PLFs, that it localizes in the cytoplasm of resting cells, and that the expression is translocated to the nuclei upon PCS stimulation. EGR plays a potentially important role in PLF growth.

• 大阪歯科学会の論文

著者

• Kakudo Kenji

  Second Department of Oral and Maxillofacial Surgery, Osaka Dental University

• Tamura Isao

  Department of Biochemistry Osaka Dental University

• Tanaka Akio

  Department of Oral Pathology, Osaka Dental University

• Ikeo Takashi

  Department of Biochemistry Osaka Dental University

• Tanaka Akio

  Department Of Oral Pathology Osaka Dental University

• Ikeo Takashi

  大阪歯科大学 生化学

• TANAKA Yoshihito

  Department of Biochemistry, Osaka Dental University

• Amakata Seiji

  Department of Biochemistry, Osaka Dental University

• Amakata Seiji

  大阪歯科大学 生化学

• Amakata Seiji

  Department Of Biochemistry Osaka Dental University

• Arika Takumi

  Department Of Oral And Maxillofacial Surgery National Hospital Organization Osaka National Hospital

• Arika Takumi

  大阪歯科大学 第二口腔外科学

• Arika Takumi

  Second Department Of Oral And Maxillofacial Surgery Osaka Dental University

• Kakudo Kenji

  大阪歯科大学 大学院歯学研究科口腔外科学第二

• Kakudo Kenji

  Second Department Of Oral And Maxillofacial Surgery Osaka Dental University

• Kakudo Kenji

  Oral And Maxillofacial Surgery (ii) Osaka Dental University

• Kakudo Kenji

  Second Department Of Oral And Maxillofacial Surgery Osaka Dental Universty

• Sakaki Toshio

  Second Department of Oral and Maxillofacial Surgery, Osaka Dental Universty

• Nishikawa Tetsunari

  Department of Oral Pathology, Osaka Dental University

• Nishikawa Tetsunari

  Department Of Oral Pathology Osaka Dental University

• Nishikawa Tetsunari

  大阪歯科大学 口腔病理学

• Sakaki Tetsuya

  Department Of Biochemistry Osaka Dental University

• Sakaki Tetsuya

  大阪歯科大学 第2口腔外科

• Sakaki T

  Second Department Of Oral And Maxillofacial Surgery Osaka Dental Universty

• Tanaka Akio

  Osaka Dental Univ. Osaka Jpn

• Sakaki Toshio

  Department Of Biochemistry Osaka Dental University

• Tanaka Yoshihito

  Department Of Biochemistry Osaka Dental University

• Sakaki Toshio

  Second Department Of Oral And Maxillofacial Surgery Osaka Dental University

• Tanaka A

  Department Of Oral Pathology Osaka Dental University

• Tanaka Akio

  大阪歯科大学 口腔病理学

• Aoki S

  大阪歯科大学 生化学

• Ikeo T

  Department Of Biochemistry Osaka Dental University

• Tanaka Akio

  Department Of Electrical Engineering Nagaoka University Of Technology

• Kakudo Kenji

  Second Department Of Oral And Maxillofacial Surgery Osak Dental University

• Tanaka Akio

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

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