Expression of early growth response-1 and -2 in human periodontal ligament fibroblasts
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概要
- 論文の詳細を見る
We investigated expression of early growth response (EGR)-1 and EGR-2 in human periodontal ligament fibroblasts (PLFs). After cultivating PLFs in Dulbecco's modified Eagle's medium (DME) for two days without 10% fetal calf serum (FCS), we incubated them in DME supplemented with FCS for 15, 30, 45, 60 and 120min. 5-bromo 2'-deoxyuridine incorporation showed a peak in the PLFs treated for 45 min. Expressions of EGR-1 protein and mRNA were seen beginning 15 min after treatment. EGR-1 protein gradually increased during this period. Anti-EGR-1 antibody strongly reacted with the nuclear region at 30 min. Maximum expression of EGR-2 protein was seen at 30 min, and it was localized in and around the nucleus. Expression of EGR-2 mRNA showed a peak at 15 min. These results demonstrate that EGR expression is more pronounced in FCS-stimulated PLFs, that it localizes in the cytoplasm of resting cells, and that the expression is translocated to the nuclei upon PCS stimulation. EGR plays a potentially important role in PLF growth.
- 大阪歯科学会の論文
著者
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Kakudo Kenji
Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
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Tamura Isao
Department of Biochemistry Osaka Dental University
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Tanaka Akio
Department of Oral Pathology, Osaka Dental University
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Ikeo Takashi
Department of Biochemistry Osaka Dental University
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Tanaka Akio
Department Of Oral Pathology Osaka Dental University
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Ikeo Takashi
大阪歯科大学 生化学
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TANAKA Yoshihito
Department of Biochemistry, Osaka Dental University
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Amakata Seiji
Department of Biochemistry, Osaka Dental University
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Amakata Seiji
大阪歯科大学 生化学
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Amakata Seiji
Department Of Biochemistry Osaka Dental University
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Arika Takumi
Department Of Oral And Maxillofacial Surgery National Hospital Organization Osaka National Hospital
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Arika Takumi
大阪歯科大学 第二口腔外科学
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Arika Takumi
Second Department Of Oral And Maxillofacial Surgery Osaka Dental University
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Kakudo Kenji
大阪歯科大学 大学院歯学研究科口腔外科学第二
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Kakudo Kenji
Second Department Of Oral And Maxillofacial Surgery Osaka Dental University
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Kakudo Kenji
Oral And Maxillofacial Surgery (ii) Osaka Dental University
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Kakudo Kenji
Second Department Of Oral And Maxillofacial Surgery Osaka Dental Universty
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Sakaki Toshio
Second Department of Oral and Maxillofacial Surgery, Osaka Dental Universty
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Nishikawa Tetsunari
Department of Oral Pathology, Osaka Dental University
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Nishikawa Tetsunari
Department Of Oral Pathology Osaka Dental University
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Nishikawa Tetsunari
大阪歯科大学 口腔病理学
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Sakaki Tetsuya
Department Of Biochemistry Osaka Dental University
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Sakaki Tetsuya
大阪歯科大学 第2口腔外科
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Sakaki T
Second Department Of Oral And Maxillofacial Surgery Osaka Dental Universty
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Tanaka Akio
Osaka Dental Univ. Osaka Jpn
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Sakaki Toshio
Department Of Biochemistry Osaka Dental University
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Tanaka Yoshihito
Department Of Biochemistry Osaka Dental University
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Sakaki Toshio
Second Department Of Oral And Maxillofacial Surgery Osaka Dental University
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Tanaka A
Department Of Oral Pathology Osaka Dental University
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Tanaka Akio
大阪歯科大学 口腔病理学
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Aoki S
大阪歯科大学 生化学
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Ikeo T
Department Of Biochemistry Osaka Dental University
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Tanaka Akio
Department Of Electrical Engineering Nagaoka University Of Technology
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Kakudo Kenji
Second Department Of Oral And Maxillofacial Surgery Osak Dental University
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Tanaka Akio
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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