Heparin-binding epidermal growth factor small interfering RNA vector restrains the invasion activity of an oral cancer cell line

概要

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Heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been shown to stimulate the growth of a variety of cells in an autocrine or paracrine manner. Although HB-EGF is widely expressed in tumors when compared to normal tissue, its contribution to tumorigenicity is still not known. We analyzed the effects of HB-EGF on the invasion activity of a cultured oral cancer cell line, HSC3, using small interfering RNA (siRNA) vector. An oral squamous carcinoma cell line, HSC3, was transfected with siRNA vector targeting HB-EGF (HSC3-siRNA). Expression of HB-EGF was analyzed by real-time polymerase chain reaction. The invasiveness of the transfected cells was determined using Matrigel Invasion Assay, and MMP-9 production was measured by reverse transcriptional polymerase chain reaction (RT-PCR) and gelatin zymography. The expression of HB-EGF was reduced in HSC3-siRNA cells. The Matrigel Invasion Assay demonstrated that invasiveness of HSC3-siRNA cells was also reduced. RT-PCR demonstrated that in HSC3-siRNA cells, MMP-9 mRNA was decreased. These findings suggest that HB-EGF expression is related to the invasion activity of oral cancer, particularly via regulation of MMP-9.