Keratin 19 in Benign Ameloblastomas and Malignant Ameloblastoma : Immunohistochemistry, Reverse Transcription : Polymerase Chain Reaction and Northern Blot Analysis

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The authors examined expression of keratin 19 by immunohistochemistry, reverse transcription-polymerase chain reaction(RT-PCR)and Northern blot analysis in benign ameloblastomas(n=10)and malignant ameloblastoma(n=1). Immunohistochemical expression of keratin was confined to the basal cells in normal gingival epithelia. in the stellate cells and squamous metaplastic cells in central nests of follicular ameloblastomas, and in peripheral cells of plexiform ameloblastomas. RT-PCR and Northern blot analysis showed that keratin 19 mRNA expression was sparse in the in gingiva, weak in plexiform ameloblastoma, moderate in follicular ameloblastoma, and intense in malignant ameloblastoma. In this study, we conclude that ameloblastoma cells that retain the characteristics of tooth germ may proliferate. The differentiation made of ameloblastoma and malignant ameloblastoma may be different from the stratification of squamous cell. Keratin 19 may not be useful as a malignant marker in ameloblastoma.
硬組織再生生物学会の論文