The ter/ter Gonadal Somatic Cells Cause Apoptosis in ter/ter Primordial Germ Cells (PGCs) with Normal Survivability and Proliferation Ability in the Mouse : Evidence from PGC-Somatic Cell "Exchange-Co-Culture"(Developmental Biology)

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概要

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• The ter (teratoma, chromosome 18) mutation causes a deficiency in primordial germ cells (PGCs) of ter/ter embryos from ter congenic mouse strains at around 8.0 days post coitum (dpc). Our previous studies indicated that in vivo, apoptosis of PGCs was caused by ter/ter gonadal somatic cells. To examine survival and proliferation in ter/ter PGCs and the deficiency caused by ter/ter gonadal somatic cells in vitro, we performed "exchange-co-culture" of PGCs and gonadal somatic cells by combining different tergenotypes, on SI/SI4-m220 feeder cells. The number of PGCs after 3 days culture of 9.5 dpc ter/ter PGCs with +/+ 12.5 dpc gonadal somatic cells increased similar to that of +/teror +/+ PGCs. The numbers of PGCs after 12 hr culture of +/+ and ter/ter 11.5 dpc PGCs with 11.5 dpc ter/ter gonadal somatic cells decreased significantly when compared to those cultured with +/+ somatic cells. PGCs preferred the WT1-positive gonadal somatic cells, Sertoli cells, to the feeder cells. Both TUNEL and BrdU assays showed that ter/ter somatic cells induced apoptosis but were independent of DNA synthesis in PGCs "exchange-co-cultured". Through these results, we demonstrated for the first time that in vitro ter/ter PGCs showed survival and proliferation activities in response to the gonadal somatic cells and that ter/ter gonadal somatic cells caused apoptosis in PGCs through cell-cell contact.

• 社団法人日本動物学会の論文
• 2001-07-01

著者

• Nozaki Masami

  Department Of Science For Animal Experimentation Research Institute For Microbial Diseases Osaka Uni

• Noguchi Motoko

  Department Of Biology And Geosciences Faculty Of Science Shizuoka University

• Nozaki Masami

  Department Of Molecular Embryology Research Institute For Microbial Diseases Osaka University

• Ishikawa Katsutoshi

  Department Of Biology And Geosciences Faculty Of Science Shizuoka University

• Takabayashi Shuji

  Department of Biology and Geosciences, Faculty of Science, Shizuoka University

• Takabayashi S

  Hamamatsu Univ. School Of Medicine Shizuoka

• NOZAKI Masami

  Department of Microbial Genetics, Research Institute for Microbial Diseases, Osaka University

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