Expression and localization of type III collagen mRNA in the healing process of rat extraction wounds

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Reticular fibers, which are almost completely type III collagen, form a scaffold for cell migration, whereas collagen fibers, which are type I collagen, are responsible for tissue support. To elucidate the role of reticular fibers in the wound healing process, we oberved tooth extraction sites in rats using the reticular silver impregnation technique, immunohistochemistry for type III collagen, and the rapid in situ hybridization (rapid ISH)method for the type III collagen gene. Type III collagen was found where the reticular fibers had been present. The expression of the type III collagen and the type III collagen gene was observed in the extraction wound after 3 days, but gradually decreased and disappeared by 14 days. Reticular fibers composed of type III collagen and the type III collagen gene were found during the granulation tissue stage of wound healing. During the process of wound healing after tooth extraction, reticular fibers were replaced with collagen fibers, and type III collagen disap-peared. These results indicate that bone is formed in the area where reticular fibers had been,and that the decreased production of type III collagen leads to the start of bone formation.
大阪歯科学会の論文