Influence of MWCNTs to Myocardial Contraction Rhythms on Differentiation of ES-D3 Cells in Three-dimensional Culture
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概要
- 論文の詳細を見る
The embryotoxicity of multi-walled carbon nanotubes (MWCNTs) has been investigated using the embryonic stem cell test (EST) protocol. Embryoid bodies (EBs) were generated with the hanging drop culture of ES-D3 cells, followed by one-week culture in three-dimensionally cultured collagen gel with MWCNTs. The contraction of myocardia that differentiated in teratomas could be microscopically observed. However, the myocardial contraction rhythms were examined by image analysis, because they markedly varied. Without MWCNTs, the contraction rhythms of the myocardia were constant. Some teratomas showed peristalsis, suggesting differentiation into the gastrointestinal tract. However, MWCNTs mixture had no effect on the peristaltic rhythms. Conductive MWCNTs that contaminate the scaffolds may have electrical effects. This phenomenon should be further investigated because biological problems are a concern regarding MWCNTs.
- ナノ・バイオメディカル学会の論文
著者
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Imai Koichi
Department Of Biomaterials Osaka Dental University
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Nishikawa Tetsunari
Department Of Oral Pathology Osaka Dental University
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Sawai Hirofumi
Department Of Internal Medicine Osaka Dental University
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WATARI Fumio
Graduate School of Dental Medicine, Department of Biomedical, Dental Materials & Engineering, Hokkai
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AKASAKA Tsukasa
Graduate School of Dental Medicine, Hokkaido University
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Okamura Tomoharu
Department Of Oral Pathology Osaka Dental University
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Tanaka Akio
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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HONDA Yoshitomo
Institute of Dental Research, Osaka Dental University, Osaka, Japan
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SHIRAI Tsubasa
Department of Biomaterials, Osaka Dental University, Osaka, Japan
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SUESE Kazuhiko
Department of Esthetic Dentistry, Osaka Dental University, Osaka, Japan
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OGAWA Fumiya
Department of Postgraduate Clinical Training, Osaka Dental University, Osaka, Japan
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TAKASHIMA Hiromasa
Ina Research Inc., Nagano, Japan
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