Gene expression analysis of cartilage-like components in pleomorphic adenoma

概要

論文の詳細を見る
Pleomorphic adenoma (PA) is the most common salivary gland tumor and presents histological features indicative of ductal structures intermingled with mucoid, myxoid or chondroid tissues. The chondroid structure consists of chondrocyte-like cells that are associated with the cartilage lacunae of the basophilic matrix. We attempted to characterize the cartilage-like components using immunohistochemistry and gene expression analysis. We demonstrated that cartilage-like components were similar to normal cartilage in histochemistry and immnohistochemistry. Among the eleven genes with gene expression profiles that were increased by more than 8-fold in patients with PA compared with those with adenoid cystic carcinoma (AdCC), the chondrogenesis- associated YKL-39 and YKL-40 genes were selected for the present study. Quantitative analysis was performed using real-time RT-PCR for those two genes. The expression levels of the two genes were higher than those of other genes with elevated expression levels in the PA patients. Our results indicated that the structure of chondroid tissue may be similar to that of true cartilage and that cartilage formation tends to be observed in tissue types with less mesenchymal structure.
2013-04-00