A Double Staining Method for Histone H3 mRNA and p53 Protein in Oral Tumors Using In Situ Hybridization and Immunohistochemistry
スポンサーリンク
概要
- 論文の詳細を見る
Proliferative and aberrant cellular activities of human tissues were evaluated using a novel double staining technique for histone H3 (with in situ hybridization) and immunohistochemistry for p53 protein. Simultaneous analyses were run on tissue sections of papillomas, squamous cell carcinomas (SCC) and normal non-neoplastic stratified squamous epithelia from human oral specimens using formalin-fixed, paraffin-embedded material. Sections were first FITC-labeled histone H3 DNA probe and then treated with alkaline phosphatase-conjugated anti-FITC rabbit antibody. Following reaction with anti-p53 protein mouse monoclonal antibody using the labeled streptavidin-biotin (LSAB) method, sections were stained with 3-amine-9-ethylcarbazole-HCI to detect p53 protein, and with 5-bromo-4-chloro-3-indoxy phos-phate/nitro blue tetrazolium to detect histone H3 mRNA. Several methods differing only in the order of detection procedures were employed and subsequently compared. Using these methods, histone H3 mRNA and p53 protein were detected both within the cytoplasm and the nucleus, respectively. Histone H3 mRNA and p53 protein were expressed in larger cell populations in the following order: SCC, papilloma, and non-neoplastic stratified squamous epithelia. The double staining as employed here proved effective for simultaneous evaluation of cell proliferative activity as well as the over-expression of aberrant gene on the same tissue sections.
- 日本組織細胞化学会の論文
著者
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Kobayashi Hideki
Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University
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Wato Masahiro
Department of Oral Pathology, Osaka Dental University
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Tanaka Akio
Department of Oral Pathology, Osaka Dental University
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Tanaka Akio
Department Of Oral Pathology Osaka Dental University
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Wato Masahiro
大阪歯科大学 口腔病理学
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Wato Masahiro
Department Of Oral Pathology Osaka Dental University
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Arika Takumi
大阪歯科大学 第二口腔外科学
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Arika Takumi
Second Department Of Oral And Maxillofacial Surgery Osaka Dental University
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Nishikawa Tetsunari
Department of Oral Pathology, Osaka Dental University
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Nishikawa Tetsunari
Department Of Oral Pathology Osaka Dental University
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Nishikawa Tetsunari
大阪歯科大学 口腔病理学
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MASUNO Kazuya
Department of Oral Pathology, Osaka Dental University
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TOMINAGA Kazuya
Department of Oral Pathology, Osaka Dental University
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MORI Masahiko
Department of Oral Pathology, Osaka Dental University
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Tominaga Kazuya
Department Of Oral Pathology Osaka Dental University
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Mori M
Department Of Oral-maxillofacial Surgery Asahi University School Of Dentistry:medicaraise Corporatio
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Mori Masahiko
Department Of Oral Pathology Osaka Dental University
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Uobe Kenichi
Department of Oral Pathology, Osaka Dental University
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Tominaga Kazuya
Osaka Dental Univ. Osaka Jpn
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Tanaka Akio
Osaka Dental Univ. Osaka Jpn
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Wato Masahiro
Osaka Dental Univ. Osaka Jpn
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Mori M
Department Of Oral And Maxillofacial Surgery School Of Dentistry Asahi University
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Mori M
Department Of Oral Surgery Asahi University School Of Dentistry
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Mori M
Department Of Oral Surgery Gifu College Of Dentistry
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ARAI Shoichi
Department of Radiological Sciences International University of Health and Welfare
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Yoshida Seiko
Department of Oral Pathology Osaka Dental University
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Mori Masahiko
Takasago Research & Development Center Mitsubishi Heavy Industries Ltd.
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Mori Masahiko
Radiation Hazards Research Group National Institute Of Radiological Sciences
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Uobe K
Department Of Oral Pathology Osaka Dental University
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Uobe Kenichi
Department Of Oral Pathology Osaka Dental University
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Masuno Kazuya
Department Of Oral Pathology Osaka Dental University
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Muto Masahiro
Department Of Oral Pathology Osaka Dental University
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Arai Shoichi
Department Of Pathology Nara Shakaihokenn Hospital
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Mori Masahiko
Department Of Oral And Maxillofacial Surgery Asahi University School Of Dentistry
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Mori Masahiko
Department Of Applied Chemistry Faculy Of Engineering And High Technology Research Center Kansai Uni
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Kobayashi Hideki
Department Of Oral Pathology Osaka Dental University
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Kobayashi Hideki
Department Of Cardiology Tokyo Women's Medical College
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Kobayashi Hideki
Department Of Cardiology Tokyo Metropolitan Fuchu Hospital
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Tanaka Akio
Department Of Electrical Engineering Nagaoka University Of Technology
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Tanaka Akio
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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