Rate of protein transfer from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose membranes
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概要
- 論文の詳細を見る
About 5μg of protein is generally needed for SDS-PAGE and about 35μg for Western blot analysis. Because purified protein is very precious, we attempted to determine the best transfer efficiency for these analyses that used the smallest amount of protein. We investigated the detection limit of albumin on gel and on nitrocellulose membrane, the transfer rate and time from an electrophoresed gel to a nitrocellulose membrane, and the detection limit of albumin for dot blot analysis and Western blot analysis. We found that the detection limit of albumin was 0.05μg on gel and 0.5μg on nitrocellulose membrane when stained with CBB. The transfer rate from gel to nitrocellulose membrane was 10% and the time was over 90 min. The detection limit of albumin was 0.3μg for the dot blot analysis and 0.5μg for Western blot analysis. Our results suggest that the amount of applied protein can be reduced to almost 1/100th for SDS-PAGE and to between 1/11th and 1/70th for Western blot analysis while still using conventional procedures.
- 大阪歯科学会の論文
著者
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YAMAMOTO Kazuyo
Department of Operative Dentistry, Osaka Dental University
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Tanaka Akio
Department of Oral Pathology, Osaka Dental University
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Tanaka Akio
Department Of Oral Pathology Osaka Dental University
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Arika Takumi
Department Of Oral And Maxillofacial Surgery National Hospital Organization Osaka National Hospital
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Arika Takumi
大阪歯科大学 第二口腔外科学
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Arika Takumi
Second Department Of Oral And Maxillofacial Surgery Osaka Dental University
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TOMINAGA Kazuya
Department of Oral Pathology, Osaka Dental University
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Yamamoto Kazuyo
Department Of Operative Dentistry Osaka Dental University
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Tominaga Kazuya
Department Of Oral Pathology Osaka Dental University
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Suwa Sayaka
Graduate School of Dentistry (Operative Dentistry), Osaka Dental University
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Kawamoto Masayuki
Department of Operative Dentistry, Osaka Dental University
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Hata Shintaro
Graduate School of Dentistry, Osaka Dental University
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Tominaga Kazuya
Osaka Dental Univ. Osaka Jpn
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Suwa Sayaka
Graduate School Of Dentistry (operative Dentistry) Osaka Dental University
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Tanaka Akio
Osaka Dental Univ. Osaka Jpn
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Kawamoto Masayuki
Department Of Operative Dentistry Osaka Dental University
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Tanaka A
Department Of Oral Pathology Osaka Dental University
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Tanaka Akio
大阪歯科大学 口腔病理学
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Hata Shintaro
Graduate School Of Dentistry (pathology) Osaka Dental University
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Hata Shintaro
Osaka Dental Univ. Osaka Jpn
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Yamamoto Kazuyo
Osaka Dental Univ. Osaka Jpn
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Tanaka Akio
Department Of Electrical Engineering Nagaoka University Of Technology
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Yasuno Kenzo
Graduate School Of Dentistry (operative Dentistry) Osaka Dental University
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Tanaka Akio
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Yamamoto Kazuyo
Department of Hematology, Shinko Hospital, Japan
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