IMMUNOHISTOCHEMICAL OBSERVATIONS OF EPIDERMAL GROWTH FACTOR, KERATIN PROTEINS AND LECTIN BINDING ON EFFECTS OF TESTOSTERONE ADMINISTRATION IN DUCT-LIGATED SUBMANDIBULAR GLANDS OF MICE

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Histologic and histochemical changes of epidermal growth factor (EGF), keratin-like immunoreative materials (KLIM) and lectin bindings (PNA, SBA and DBA) were reported in duct-ligated submandibular glands (SMGs) of mice with varying treatments of testosterone and with castration or both. Testosterone treatment before duct ligation of SMGs was markedly influenced, and EGF staining in altered granular convoluted tubule (GCT) cells showed highly positive as in the normal GCT cells. Testosterone treatment after duct ligation as well as hormone treatments before sacrifice were also done. Testosterone effects on duct-ligated SMGs were evident in degranulated GCT cells and duct-like structures and in enhanced secretory granules of degenerated ductal epithelia. These changed GCT cell's findings were observed in EGF reaction, and lectin bindings for PNA and SBA. Keratins (KLIM) in duct-ligated SMGs appeared in duct-like structures, whereas they decreased in proportion to the number of secretory granules after hormone treatment. Testosterone effects on GCT cells and degranulated tubules appeared till the 7th day after injection, and were not evident at the 2nd and 3rd weeks, irrespective of different treatment methods: with pre-ligated testosterone and post-ligated groups and with castration.
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