HETEROGENEITY OF KERATIN DISTRIBUTION AS REVEALED BY MONOCLONAL ANTIBODIES IN SALIVARY PLEOMORPHIC ADENOMAS

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Eighty pleomorphic adenomas of human salivary glands were examined by the immunoperoxidase method using a variety of monoclonal antibodies specific for certain of the cytokeratins. Expressions of monoclonal antibodies against KL1, PKK1, K8.13, K8.60, K4.62, RPN1164 and RPN1165 were observed in luminal tumor cells of pleomorphic adenomas, as well as in intercalated and striated duct cells of the normal salivary glands, while monoclonal antibody K8.12 was reacted to outer tumor cells, as well as ductal basal cells in the normal glands. According to keratin existence with the use of monoclonal antibodies, outer tumorcells of tubulo-ductal structures contained keratin nos. 16 and 13, and luminal tumor cells nos. 19, 18, 11, 10 and 8, Great heterogeneities of keratin distribution were given in outer and luminal tumor cells of tubulo-ductal and duct-like structures. It is postulated that stem cells may be present in intercalated ductal segments and they differentiate to both ductal basal cells or intercalated ductal cells, and two cells showed different expressions for keratins. Pleomorphic adenoma might arise from those two progenitor cells, is suspected. The presence of reserve cells may coincide with progenitor cells as ductal basal cells, and ductal basal cells may differentiate to outer tumor cells and also modified myoepitheial cells.
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