IMMUNOHISTOCHEMICAL DEMONSTRATION OF S-100 PROTEIN IN CARTILAGE TISSUE INDUCED BY ELECTRIC STIMULATION OR BY BONE MORPHOGENETIC PROTEIN

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Chondroidal tissue induced by electric stimulation in rat tibial periosteum or by bone morphogenetic protein (BMP) implanted in mouse thigh muscle was examined immunohistochemically for S-100 protein with the use of polyclonal antiserum (S-100) and monoclonal antibodies to S-100 protein subunits α and β (S-100α and S-100β). Chondrocytes were occasionally found in the electric callus (EC), and ossification induced by BMP began from chondroidal tissue in the muscle. All the types of cartilage cells displayed strong staining for S-100 and S-100α subunit, and slight staining for S-100β. Bone cells such as osteocytes and osteoclasts appearing after chondrogenesis were generally devoid of S-100 protein. During bone formation, the immunoreaction for S-100 protein was characteristically restricted to cartilage cells. The presence of S-100 protein throughout chondrogenesis suggests that this protein might participate in calcium-protein interactions occurring during cartilage formation.
日本組織細胞化学会の論文